Presented at: 107th Annual Meeting of the American Association for Cancer Research, New Orleans, LA, April 16-20, 2016.
Published in: Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC
Breast cancer remains a leading cause of women’s death worldwide with progressive emergence of resistance towards approved chemotherapies. For this reason, new complementarytreatments have been sought. One of those includethe use of a phytobiological mixture against different types of malignancies, including but not limited to breast cancer. Herein, we investigated the anti-proliferative effects of a novel phytobiological mixture (PB)against two breast cancer cell lines :MDA-MB-231 and MCF-7.The PB mixture is composed of the following: quercetin, resveratrol, cruciferex, epigallocatechin-3-gallate and curcumin at different proportions. Theanti-proliferative and pro-apoptotic effects of PB were evaluated at non-cytotoxic concentrations as determined usingthe Sulfa-Rhodamine B assay (SRB).The effects of PB on the induction of apoptosis were valuated using Annexin-V and flow cytometry to measure the resultingDNA fragments. The results showed that at 72hrs, PB had cytotoxic effects against MDA-MB-231 and MCF-7 cells with an IC50’s of 25.8±5.4 µg/ml and 56.9±4.2 µg/ml, respectively. Exposure of MDA-MB-231 to PB for 72hrs resulted in the induction of apoptosis in a dose-dependent manner. PB concentrations of 10 µg/ml and 30 µg/ml, resulted in early programmed cell death of 9.1±1.5% and 22.1±1.8% respectively. Exposure to PB (10 µg/ml for 24 hrs) resulted in mild cell cycle arrest at the G2/M phase20.3±0.3% ;while higher dosesshowed a significant cell cyclearrest at theS-phaseas compared to the control untreated cells (p<0.001). However, exposure to PB for 48 hrs at both doses,10 µg/ml and30 µg/ml,resulted in a significant cell cycle arrest at the S-phase 25.3±1.6% and 20.5± 0.4%respectivelyas compared to the control untreated cells 12.4±1.5% (p<0.001). In conclusion, PB was found to have a promising anti-proliferative and pro-apoptotic effect in breast cancer cell lines. Further studies are needed to address the underlying mechanism of action of PB against breast cancer cell lines.
Afnan A. Alqarni, Aliaa A. Alamoudi, Ghada M. Ajabnoor, Ahmed M. Al-Abd, Aleksandra Niedwiecki, Matthias Rath, Steve Harakeh. Inhibition of proliferation and induction of apoptosis by a novel phytobiological mixture against breast cancer cell lines [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 4208. doi:10.1158/1538-7445.AM2017-4208
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