Inhibition of Pulmonary Metatasis of Melanoma B16FO Cells in C57BL/6 Mice by a Nutrient Mixture Consisting of Ascorbic Acid, Lysine, Proline, Arginine, and Green Tea Extract

M.W. Roomi, N. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Experimental Lung Research 2006, 32(10):517-530.


Tumor metastasis is a major reason for treatment failure in cancer patients. Melanoma is one of the types of cancer that most frequently metastasize, especially in the lung. Currently used therapies are ineffective with a high level of toxicity and mortality.Cancer metastasis is accompanied by degradation of connective tissue necessary to facilitate the spread and invasion of cancer cells from one organ to other parts of the body.

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Suppression of growth and hepatic metastasis of murine B16FO melanoma cells by a novel nutrient mixture

M.W. Roomi, T. Kalinovsky, N.W. Roomi, V. Ivanov, M. Rath M, A. Niedzwiecki
Oncology Reports 2008; 20(4): 809-817

Highly metastatic melanoma is resistant to existing therapies. Our main objective was to investigate the effect of a nutrient mixture (NM) on B16FO tumor growth and hepatic metastasis. Tumor growth was studied in athymic nude male mice, 5-6 weeks old, inoculated with 106 B16FO melanoma cells subcutaneously and fed either a regular diet or one supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. Metastasis was studied in C57BL/6 mice, which received 106 B16FO melanoma cells by intrasplenic injection and a regular diet or one supplemented with NM 0.5% for 2 weeks.

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A nutrient mixture suppresses hepatic metastasis in athymic nude mice injected with murine B16FO melanoma cells


M.W. Roomi, T. Kalinovsky, N.W. Roomi, J. Monterrery, M. Rath, A. Niedzwiecki
BioFactors 2008, 33(3): 181-189

Highly metastatic melanoma is resistant to existing therapies. A unique micronutrient mixture (NM) containing ascorbic acid, amino acids, green tea extract has been shown to exhibit anticancer activity in vivo and in vitro in a number of cancer cell lines including human and murine melanoma cells lines. In this study we examined the effect of dietary NM supplementation on hepatic metastasis of intrasplenic injection of B16FO melanoma cells in athymic nude mice. Athymic nude mice (n=10), 10-12 weeks of age, received 106 B16FO melanoma cells by injection into the spleen and divided into two groups.

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Micronutrient Synergy – a New Tool in Effective Control of Metastasis and Other Key Mechanisms of Cancer

A. Niedzwiecki, M.W. Roomi, T. Kalinovsky, M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050

Cancer and Metastasis Reviews 2010; 29(3): 529-542

Consumption of a plant-based diet has been associated with prevention of the development and progression of cancer. We have developed strategies to inhibit cancer development and its spread by targeting common mechanisms used by all types of cancer cells that decrease stability and integrity of connective tissue. Strengthening of collagen and connective tissue can be achieved naturally through the synergistic effects of selected nutrients, such as lysine, proline, ascorbic acid and green tea extract (NM). This micronutrient mixture has exhibited a potent anticancer activity in vivo and in vitro in a few dozen cancer cell lines. Its anti-cancer effects include inhibition of metastasis, tumor growth, MMP secretion, invasion, angiogenesis and cell growth as well as induction of apoptosis

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