Gastrointestinal Cancers

In Vitro Effect of Cytokines, Inducers, and Inhibitors on the Secretion of MMP-2 and MMP-9 in Hepatocarcinoma Cell Line SK-Hep-1

Roomi MW, Kalinovsky T, Bhanap B, Niedzwiecki A, Rath M

Integrative Cancer Therapies Volume 18: 1–12, 2019 DOI: 10.1177/1534735419889155



The prognosis of hepatocellular carcinoma (HCC) remains dismal despite any treatment. Matrix metalloproteinases (MMPs) have been researched for their role in tumor invasion and metastasis. Various cytokines, mitogens, growth factors, inducers, and inhibitors control MMP activities. In this article, we investigated the roles of these in the regulation of MMP2, -9 secretions in HCC. Human HCC SK-Hep-1 was grown in appropriate media. At near confluence, the cells were washed with phosphate-buffered saline and incubated in serum-free media with PMA; TNF-α, IL-1β; lipopolysaccharide; epigallocatechin gallate (EGCG) and doxycycline (Dox) at various doses with and without PMA; a nutrient mixture (NM) containing lysine, proline, ascorbic acid, and EGCG with and without PMA at; and actinomycin D and cycloheximide at different doses. After 24 hours, the media were removed and analyzed. SK-Hep-1 expressed bands corresponding to MMP-2 and MMP-9. TNF-α showed an insignificant effect on MMP-2 at doses below 25 at which dose MMP-2 was virtually blocked and a moderate dose-dependent effect on MMP-9.

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Antitumor effect of a nutrient mixture on colon cancer cells

M. W. Roomi, B. Bhanap, A. Niedzwiecki, M. Rath

Journal of Cellular Medicine and Natural Health, July 2019



Colorectal cancer is the second most deadly cancer in the United States and there is an urgent need to develop effective preventive and therapeutic strategies against it. The aim of our study was to investigate the antitumor effects of a nutrient mixture containing ascorbic acid, lysine, proline and green tea extract that has been previously demonstrated a strong anticancer activity in several cancer cell types. We studied the effect of NM on human colon cancer cell line HCA-7 in vitro by measuring cancer cell proliferation, invasion, and expression of pro-inflammatory COX-2 and COX-1 proteins.

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Antitumor Effect of a Combination of Lysine, Proline, Arginine, Ascorbic Acid, and Green Tea Extract on Pancreatic Cancer Cell Line MIA PaCa-2

M.W.Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
International Journal of Gastrointestinal Cancer 2005, 35(2): 97-102.

Current treatment of pancreatic cancer is generally associated with poor prognosis, even if diagnosed early, due to aggressive rate of metastasis and non-responsiveness to chemotherapy and radiotherapy. Matrix metalloproteinases (MMPs) have received much attention in recent years for their role in various malignancies, and have been implicated in tumor invasion, metastasis and angiogenesis.

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Modulation Of Upa, Mmps And Their Inhibitors By A Novel Nutrient Mixture In Human Colorectal, Pancreatic And Hepatic Carcinoma Cell Lines

Roomi MW, Kalinovsky T, Niedzwiecki A, Rath M

Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
International Journal of Oncology –2015; 47(1): 370-376


Colorectal, pancreatic and hepatic carcinomas are characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the ECM and basement membrane, allowing cancer cells to spread to distal organs. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of uPA and MMPs with cancer progression, metastasis and shortened patient survival.

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Synergistic Effect of Combination of Lysine, Proline, Arginine, Ascorbic Acid and Epigallocatechin Gallate on Colon Cancer Cell Line HCT 116

M.W. Roomi, V.Ivanov, T.Kalinovsky, A. Niedzwiecki, M.Rath
Journal of the American Nutraceutical Association 2004, 7(2): 40-43

Limitation of current treatment methodologies to control metastasis, as well as the proposed antitumor properties of specific nutrients prompted us to investigate the effect of a specific formulation (NS) of lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on human colon cancer cells HCT 116 for viability, MMP expression, invasion, and morphology.

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