Angiogenesis

M.W. Roomi, N. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Oncology Reports 2005, 14(4): 807-815

Introduction:

Degradation of extracellular matrix (ECM) is a hallmark of tumor invasion, metastasis and angiogenesis. Based on Rath multitargeted approach to cancer by using natural substances to control ECM stability and enhancing its strength we developed a novel formulation (NM) of lysine, proline, ascorbic acid and green tea extract that has shown significant anti-cancer activity against a number of cancer cell lines. The aim of the present study was to determine whether NM exhibits anti-angiogenic and antimetastatic effects using in vitro and in vivo experimental models.

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
In Anti-Angiogenic Functional and Medicinal Foods, 2007, Losso JN, Shahidi F, Bagchi D (eds), CRC Press, Boca Raton, London, New York, 561-580.

Angiogenesis, the formation of new capillaries from existing blood vessels, is necessary for tumor growth and metastasis to distal organs. MMPs have been recognized as critical to this process secondary to their ability to digest basement membrane and ECM components. The prevention of ECM degradation through the inhibition of MMP activity and increasing its integrity and strength has been shown to be a promising therapeutic approach to block the invasion that occurs during angiogenesis. We developed a novel formulation of lysine, proline, ascorbic acid and green tea extract (NM) which has shown significant anti-cancer activity against a number of cancer cell lines. Our objective was to determine whether NM exhibits anti-angiogenic and antimetastatic effects using in vitro and in vivo experimental models. Since angiogenesis depend on interaction between tumor and endothelial cells, the present study was aimed to determine the effect of NM on both these cells types.

M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath
Experimental Oncology 2009; 31(4): 214-219

Abstract:
The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant anti-angiogenic and anti-tumor effect against a number of cancer cell lines. Using a mouse hemangioendothelioma model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating viability, MMP secretion, invasion, morphology and apoptosis.