Skin Cancer

M. W. Roomi, T. Kalinovsky, N. Jariwalla, S. Siddiqui, A. Niedzwiecki, M. Rath

Journal of Cellular Medicine and Natural Health, Mar 2017

Use of natural health products to prevent, inhibit and reverse carcinogenesis is gaining increasing importance, since scientific evidence shows that high consumption of fruits and vegetables are associated with a reduced risk of various cancers. PB, which is composed of quercetin, cruciferexTM, curcumin, green tea extract and resveratrol, was formulated by defining critical physiological targets in cancer progression and metastasis. We investigated the effect of PB on two different aggressive cancer cell lines – human fibrosarcoma cell line HT-1080 and melanoma A-2058 – by evaluating cell viability, MMP secretion, invasion and morphology. Human fibrosarcoma HT-1080 and melanoma A-2058 cell lines were obtained from ATCC and cultured in DMEM media, supplemented with 10% FBS and antibiotics in 24 well tissue plates. At near confluence, cells were treated with PB at 0, 10, 25, 50, 75 and 100 μg/ml, in triplicate at each dose. Cells were also treated with phorbol 12-myristate 13-acetate (PMA) 100 ng/ml for MMP-9 induction. Cell proliferation was assayed by MTT assay, MMP by zymography, invasion through Matrigel and morphology by H&E staining. PB significantly inhibited proliferation of fibrosarcoma HT-1080 and melanoma A-2058 cells in a dose-dependent manner with 80% (p=0.0001) inhibition at 50-100 μg/ml concentration in HT-1080 cells and ~80% (p<0.0001) at 25-100 μg/ml in A-2058 cells.

M. Waheed Roomi, Bilwa Bhanap, Aleksandra Niedzwiecki, Matthias Rath
Dr. Rath Research Institute, Santa Clara, California, USA
Integrative Cancer Therapies Volume 18: 1–8 DOI: 10.1177/1534735419832365

Abstract:
Tumor metastasis is a major cause for most cancer-related deaths. Melanoma is serious cancer that metastasizes to other areas of the body, including the lungs, liver, brain, bones, or lymph nodes. Currently used cancer therapies are ineffective with a high degree of toxicity and patient mortality. Thus, any successful treatment for melanoma must target metastasis.

Waheed Roomi, Tatiana Kalinovsky, Aleksandra Niedzwiecki, Matthias Rath
Dr. Rath Research Institute, Santa Clara, California, USA
Oncology ReportsDOI: 10.3892/or.2017.5597

Abstract:
Melanoma, an extremely aggressive cancer, causes the most skin cancer-related deaths, due to metastasis to other areas of the body, such as lymph nodes, lungs, liver, brain or bone. It is characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the extracellular matrix and basement membrane, allowing cancer cells to spread to distal organs.

M.W. Roomi, N. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
In Vivo 2006; 20(1):25-32

Introduction:
Melanoma, a very serious form of skin cancer, causes the most skin cancer-related deaths, due to metastasis. Structural changes in the ECM are necessary for cell migration during tissue remodeling. MMPs, VEGF, Ki 67 (proliferative protein), and constituents of ECM play a critical role in angiogenesis, and are crucial in neoplastic invasion and metastasis. Based on antitumor properties of certain nutrients, we investigated the effect of a diet containing lysine, proline, arginine, ascorbic acid, and green tea extract on the growth of tumors induced by implanting human melanoma A2058 cells in athymic nude mice and on the expression of MMPs, VEGF, Ki 67 and fibronectin in these tumors, as well as the production of mucin (by PAS staining).