M. W. Roomi, T. Kalinovsky, N. Jariwalla, S. Siddiqui, A. Niedzwiecki, M. Rath
Journal of Cellular Medicine and Natural Health, Mar 2017
Use of natural health products to prevent, inhibit and reverse carcinogenesis is gaining increasing importance, since scientiﬁc evidence shows that high consumption of fruits and vegetables are associated with a reduced risk of various cancers. PB, which is composed of quercetin, cruciferexTM, curcumin, green tea extract and resveratrol, was formulated by deﬁning critical physiological targets in cancer progression and metastasis. We investigated the effect of PB on two different aggressive cancer cell lines – human ﬁbrosarcoma cell line HT-1080 and melanoma A-2058 – by evaluating cell viability, MMP secretion, invasion and morphology. Human ﬁbrosarcoma HT-1080 and melanoma A-2058 cell lines were obtained from ATCC and cultured in DMEM media, supplemented with 10% FBS and antibiotics in 24 well tissue plates. At near conﬂuence, cells were treated with PB at 0, 10, 25, 50, 75 and 100 μg/ml, in triplicate at each dose. Cells were also treated with phorbol 12-myristate 13-acetate (PMA) 100 ng/ml for MMP-9 induction. Cell proliferation was assayed by MTT assay, MMP by zymography, invasion through Matrigel and morphology by H&E staining. PB signiﬁcantly inhibited proliferation of ﬁbrosarcoma HT-1080 and melanoma A-2058 cells in a dose-dependent manner with 80% (p=0.0001) inhibition at 50-100 μg/ml concentration in HT-1080 cells and ~80% (p<0.0001) at 25-100 μg/ml in A-2058 cells.