Head & Neck Cancers

M W Roomi, B Bhanap, A Niedzwiecki and M Rath

J Otolaryngol Rhinol 2017, 3:029; Vol 3 | Issue 1

Abstract:

Head and neck squamous cell carcinoma (HNSCC) is a devastating disease with poor prognosis. Matrix metalloproteinases (MMPs), specifically, MMP-2 and MML-9 play a critical role in tumorigenesis by their ability to modify tumor microenvironments, facilitating cell invasion and metastasis.

M.W. Roomi, T. Kalinovsky, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental Oncology 38(1):1-3, 2016

Abstract: 

Brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and ECM components. We previously demonstrated the effectiveness of a nutrient mixture containing ascorbic acid, lysine, proline, and green tea extract in vitro:  on activity of urokinase plasminogen activator, matrix metalloproteinases and TIMPs in various human glioblastoma (LN-18, T-98G and A-172) cell lines and on glioblastoma A-172 cell proliferation and Matrigel invasion.

M.W. Roomi, T. Kalinovsky, N.W. Roomi, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
International Journal of Oncology 2015 DOI: 10.3892/ijo.2015.2895

Abstract: 
Head and neck squamous cell carcinoma (HNSCC) and acute myeloid leukemia are the major causes of mortality and morbidity in Fanconi anemia (FA) patients. The objective of this study was to investigate the antineoplastic activity of PB, a nutrient mixture containing quercetin, curcumin, green tea, cruciferex, and resveratrol, on human FA HNSCC in vitro and in vivo. Human FA HNSCC cell line OHSU-974 (Fanconi Anemia Research Fund) was cultured in RPMI medium supplemented with 20% FBS and antibiotics. At near confluence, cells were treated in triplicate with different concentrations of PB: 0, 10, 25, 50, 75 and 100 µg/ml.

M.Waheed Roomi, Tatiana Kalinovsky, Aleksandra Niedzwiecki* and Matthias Rath
Dr. Rath Research Institute, Santa Clara, CA
International Journal of Oncology 2014; 45: 887-894

Abstract:
Brain tumors are highly aggressive tumors that are characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the extracellular membrane (ECM) and basement membrane, allowing cancer cells to spread to distal organs. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of urokinase plasminogen activators (uPA) and MMPs with cancer progression, metastasis and shortened patient survival. MMP activities are regulated by specific tissue inhibitors of metalloproteinases (TIMPs). Our main objective was to study the effect of a nutrient mixture (NM) on activity of uPA, MMPs and TIMPs in various human gliomas.