M.W. Roomi, V. Ivanov, A. Niedzwiecki, M. Rath
Third International Kidney Cancer Conference, Chicago, Illinois, November 12-14, 2004.
Third International Kidney Cancer Symposium proceedings, Abstract #40, pg 40.
Renal cell carcinoma invasion is associated with its ability to degrade the extracellular matrix by local production of gelatinase enzymes (MMP-2 and MMP-9). Although many studies on renal carcinoma have demonstrated the importance of MMPs, very little information is currently known regarding the effect of inhibitors and inducers of MMPs. We studied the effect of inducers (PMA, TNF-alpha, IL-1 beta, and LPS) and inhibitors (EGCG, doxycycline, actinomycin D, cyclohexamide, retinoic acid, dexamethasone and H-7) on RCC 786-0 MMP expression. Our results indicate that MMP-2 expression was not influenced by inducers such as PMA, cytokines (IL-1b, TNFa), and LPS; the induction of MMP-9 was associated with the specific inducer used: highest with PMA, intermediate with cytokines, and none with LPS. The inhibitors tested affected both MMP-2 and MMP-9 expression. Further studies are in progress to confirm the role of MMP-9 on Matrigel invasion using PMA, cytokines and LPS.