M.W. Roomi, N.W. Roomi, V. Ivanov, A. Niedzwiecki and M. Rath
Matthias Rath Research, Cancer Division, 1260 Memorex Drive, Santa Clara, CA 95050
1st International Symposium on Cancer Metastasis and Lymphovascular System, San Francisco, CA, April 28-30, 2005
1st International Symposium on Cancer Metastasis and Lymphovascular System: Basis for Rational Therapy, Abstract #18
Endothelial cell proliferation, migration and subsequent invasion of the underlying stoma is critical to angiogenesis. We studied the effects of a nutrient mixture (NM) of lysine, proline, arginine, ascorbic acid, and green tea extract on angiogenic parameters of human umbilical endothelial cells (HUVECs). NM showed dose-dependent inhibition of MMP-2, Matrigel invasion, cell migration, and vascular tube with complete blockage of all parameters at 500 µg/ml. NM did not significantly decrease cell proliferation or morphology. The results are important as they indicate that NM is an effective anti-angiogenic agent with no apparent cell toxicity.