Anti-Angiogenic Activity of a Novel Nutrient Mixture of Lysine, Proline, Ascorbic Acid and Green Tea Extract

M.W. Roomi, N.W. Roomi, V. Ivanov, A. Niedzwiecki and M. Rath
Matthias Rath Research, Cancer Division, 1260 Memorex Drive, Santa Clara, CA 95050

Presented at: 
1st International Symposium on Cancer Metastasis and Lymphovascular System, San Francisco, CA, April 28-30, 2005

Published in: 
1st International Symposium on Cancer Metastasis and Lymphovascular System: Basis for Rational Therapy, Abstract #18


Endothelial cell proliferation, migration and subsequent invasion of the underlying stoma is critical to angiogenesis. We studied the effects of a nutrient mixture (NM) of lysine, proline, arginine, ascorbic acid, and green tea extract on angiogenic parameters of human umbilical endothelial cells (HUVECs). NM showed dose-dependent inhibition of MMP-2, Matrigel invasion, cell migration, and vascular tube with complete blockage of all parameters at 500 µg/ml. NM did not significantly decrease cell proliferation or morphology. The results are important as they indicate that NM is an effective anti-angiogenic agent with no apparent cell toxicity.