Down Regulation of Matrix Metalloproteinases and Inhibition of Cell Invasion by a Novel Nutrient Mixture in Human FA Fibroblast Cell Lines: in vitro Study

M.Waheed. Roomi, Vadim. Ivanov, Aleksandra. Niedzwiecki and Matthias Rath
Matthias Rath Research Institute, Cancer Division, Santa Clara, CA

Presented at: 
17th Annual Fanconi Anemia Scientific Symposium, Geneva, Switzerland, September 29-October 2, 2005

Published in: 
17th Annual Fanconi Anemia Scientific Symposium Proceedings, Poster #110



Fanconi anemia (FA is characterized by a high probability of developing malignancies at an early age. Extracellular matrix (ECM) matrix metalloproteinases (MMPs) produced by tumor and stromal cells, in particular MMP-2 and MMP-9, play a key role in tumor invasion and metastasis. Our in vitro studies demonstrated that the nutrient mixture (NM) of ascorbic acid, lysine, proline, and green tea extract inhibited FA cell MMP expression and invasion - important parameters for cancer prevention. These results are significant as they indicate that the nutrient mixture has strong potential as a non-toxic treatment strategy in FA neoplasia.