Anti-Angiogenic Effect of Nutrient Synergy on Human Synovial Sarcoma Cell Line SW 982

Roomi MW, Ivanov V, Niedzwiecki A, Rath M.

Presented at: 
International Research Conference on Food, Nutrition and Cancer in Washington DC, July 15-16, 2004.

Published in: 
Conference Proceedings, pg. 31, Abstract #29.


Standard treatment of synovial sarcoma, a soft tissue with a high (50%) metastatic rate has met with poor results. In this study, we investigated the inhibitory effect of a unique nutrient mixture (NS) containing lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on metastatic potential of human synovial sarcoma cells SW 982, by measuring MMP expression and Matrigel invasion in cell culture. We found NS to inhibit the expression of both MMP-2 and -9 in a dose-dependent fashion with virtual total inhibition of MMP-2 at 500 µg/ml and MMP-9 at 50 µg/ml concentration of NS. The invasion of human synovial sarcoma cells through Matrigel was significantly reduced at 500 µg/ml (79%) and totally inhibited at 1000 µg/ml concentration of the synergistically acting nutrient mixture (p<0.0001). These results are important as they demonstrate that NS is an excellent candidate for treatment of synovial sarcoma by inhibiting critical steps in metastasis.