Suppression of Human Rheumatoid Synovial Fibroblast MMP-2 Expression and Marix Invasion by Nutrient Synergy

M.W. Roomi, V. Ivanov, A. Niedzwiecki and M. Rath
Matthias Rath Research, Cancer Research Division, Santa Clara, CA 95050

Presented at: 
9th World Congress of the Osteoarthritis Research Society International, Chicago, Illinois, December 2-5, 2004.

Published in: 
Osteoarthritis and Cartilage, Abstract #193.


In patients with rheumatoid arthritis and other inflammatory diseases, MMPs are expressed in synovial membranes and have been implicated in synovial tissue invasion into adjacent cartilage and bone. Standard anti- arthritic drugs, used for prolonged periods, have been associated with serious side effects. We studied the in vitro effect of a mixture of nutrients containing ascorbic acid, lysine, arginine and epigallocatechin gallate on human synovial fibroblast growth, MMP expression, and invasive potential. Our results demonstrated complete inhibition of MMP-2 expression and invasive potential of synovial fibroblasts at 500 _g/ml, suggesting this nutrient mixture has excellent potential as a safe anti-inflammatory agent.