Suppression of MMP Expression and Invasion of Human Cervical Cancer Cell Lines Hela and DoTc2 4510 by Nutrients

M.W. Roomi, V. Ivanov, A. Niedzwiecki, M. Rath

Presented at: 
44th Annual Meeting of the Society of Toxicology, New Orleans, March 6-10, 2005.

Published in: 
Proceedings of the 44th Annual Meeting of the Society of Toxicology, Abstract #149


Cervical cancer is the seventh most common cancer worldwide and the second most common cancer in women. Untreated dysplasia and carcinoma in situ leads to 30% to 40% invasion within ten years. We studied the synergistic effect of a unique nutrient formulation containing lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on metastatic parameters on human cervical cancer cells such as effect on MMP-2 and –9 expression and cancer cell invasive potential. Our results demonstrated complete inhibition of Hela MMP-2 expression at 1000 µg/ml and MMP-9 at 500 µg/ml, and of Matrigel invasion at 500 µg/ml. NM demonstrated complete inhibition of cervical DoTc2 4510 cell expression of MMP-9 at 500 µg/ml and of invasion at 1000 µg/ml. These findings are significant as they suggest that the nutrient mixture would be an effective and safe therapeutic regimen for treatment of cervical cancer.