Aim: In this study, we investigated the effects of a specific nutrient mixture, containing ascorbic acid, lysine, proline, green tea extract among others, in most aggressive forms of non-Hodgkin’s lymphoma — Burkitt’s lymphoma, and T-cell lymphoma — using Raji and Jurkat cells respectively. Methods:Nutrient mixture (NM) doses of 0, 10, 50, 100, 500, 1000 µg/ml, were used to study effects on cell proliferation, expression of matrix metalloproteinase, Matrigel™ invasion and apoptosis. Results:The results demonstrated that the dose response toxicity of the nutrient mixture on Raji cells gradually increased with increasing concentration. The nutrient mixture was non-toxic to Jurkat cells, however exhibited anti-proliferative properties at higher concentrations. Zymography demonstrated, NM had a significant inhibitory effect on matrix metalloproteinase-9 expression with total inhibition at 1000 µg/ml for Raji cells and at 500 µg/ml for Jurkat cells. The NM at 100 µg/ml completely inhibited Matrigel™ invasion for Raji cells, and at 1000 µg/ml for Jurkat cells. After the NM challenge virtually all Raji and Jurkat cells exposed to 1000 µg/ml were in late apoptosis. Conclusion:Considering the lack of treatment options and continually increasing incidence, NM could be further explored for its therapeutic potential in Burkitt’s lymphoma and T-cell lymphoma.
Key words: Burkitt’s lymphoma, Raji cells, Jurkat T cells, MMP, apoptosis, nutrient mixture