Essential nutrients suppress inflammation by modulating key inflammatory gene expression

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LPS inflammatory effects were analyzed in human U937 macrophages by cytokine release, cyclooxygenase (COX) enzymatic activity, COX protein expression (Western blot), specific mRNA levels (RT-PCR), and nuclear factor kappa beta (NFkB) activation (phosphorylated p65 immunoassay). Nutrient supplementation in mice altered LPS-induced cytokine response in a manner similar to ibuprofen (r=0.4157, p=0.139). Cytokine response to LPS in cultured macrophages was similar to in vivo study (r=0.718, p=0.023). NM inhibited COX2 enzymatic activity and COX2 and pro-inflammatory cytokine protein expressions were downregulated by NM at transcription level complementing a blockade in NFkB activation. NM demonstrated strong beneficial effects in experimental inflammation by targeting multiple responsible mechanisms in a complex process of inflammatory reaction to pathogens.

The full study is available online at:
http://www.spandidos-publications.com/ijmm/22/6/731

V. Ivanov, J. Cha, S. Ivanova, T. Kalinofsky, M.W. Roomi, M. Rath, A. Niedzwiecki
International Journal of Molecular Medicine 2008; 22(6): 731-741

We investigated the effects of a nutrient mixture (NM) including ascorbic acid, quercetin, naringenin, hesperetin, tea catechins, lysine, proline, arginine and N-acetyl cysteine in experimental in-vivo and in-vitro inflammation triggered by bacterial lipopolysaccharide (LPS). BALB/c mice (n=36) were supplemented with NM (200 mg/kg BW) or ibuprofen (20 mg/kg BW) for two weeks. Blood plasma, collected three hours after a single intraperitoneal injection with LPS (1 mg/kg BW), was analyzed with 14 cytokine microarray.