Inhibition of Matrix Metalloprotenase-2 Secretion and Invasion by Human Ovarian Cancer Cell Line SK-OV-3 with Lysine, Proline, Ascorbic Acid, and Green Tea Extract

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Journal of Obstetrics and Gynaecology Research 2006, 32(2):148-154.

Introduction:
Based on the poor prognosis associated with ovarian cancer and reported anti-cancer properties of specific nutrients, we investigated the effect of a nutrient mixture (NM) containing lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on human ovarian cancer cells SK-OV-3 by measuring: cell proliferation, modulation of MMP-2 and –9 secretion, and cancer cell invasive potential.

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Suppression of Human Cervical Cancer Cell Lines Hela and DoTc2 4510 MMP Expression and Matrigel Invasion by a mixture of lysine, proline, ascorbic acid, and green tea extract

M.W. Roomi, V. Ivanonv, T. Kalinovsky, A. Niedzwiecki, M. Rath
International Journal of Gynecological Cancer 2006, 16(3): 1241-1247

Introduction:
Cervical cancer, the second most common cancer in women, once metastasized, leads to poor prognosis. We investigated the antitumor effect of a mixture (NM) containing lysine, proline, arginine, ascorbic acid, and green tea extract on human cervical cancer cells Hela (CCL2) and DoTc2 4510.

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Modulation of u-PA, MMPs and their inhibitors by a novel nutrient mixture in human female cancer cell lines

M.W. Roomi, T. Kalinovsky, M. Rath and A. Niedzwiecki
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Oncology Reports 2012, 28:768-776

Abstract:
Cancers of the breast, cervix, uterus and ovary are the most prevalent cancers in women worldwide. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of urokinase plasminogen activators (u-PA) and matrix metalloproteinases (MMPs) with cancer progression, metastasis and shortened patient survival. MMP activities are regulated by specific tissue inhibitors of metalloproteinases (TIMPS).

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Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice

M.W. Roomi, T. Kalinovsky, J. Cha, N.M. Roomi,  A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental & Therapeutic Medicine 2014, DOI: 10.3892/etm.2014.2127

Abstract:
Although fully treatable in the early stages, once cervical cancer has metastasized, patient outcome is poor. The main objective of this study was to examine the effect of dietary supplementation with a nutrient mixture (NM) containing lysine, ascorbic acid, proline, green tea extract and other micronutrients on HeLa cell xenografts in nude female mice. Tumor growth was measured and immunohistochemical staining was evaluated for the following cancer markers: Ki67 (proliferation); matrix metalloproteinase (MMP) 2 and  9 (invasion/metastasis); vascular endothelial growth factor (VEGF) (angiogenesis); terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and B-cell lymphoma 2 (Bcl-2) (apoptosis); cyclooxygenase 2 (COX 2) and inducible nitric oxide synthase (iNOS) (inflammation); and glutathione S-transferase π (GSTπ) (a general cancer marker).

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Anticancer Effects of a Specific Mixture of Nutrients in Multidrug Resistant Human Uterine Sarcoma Cell Line MES-SA/Dx5 and in Drug Sensitive MES-SA Cell Line

 

M.W. Roomi, T. Kalinovsky, N.W. Roomi, M. Rath and A. Niedzwiecki
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Oncology Reports 2011, 27: 17-27

Abstract:
A specific nutrient mixture containing lysine, proline, ascorbic acid and green tea extract (NM) has demonstrated a broad spectrum of antitumor activity against a number of cancer cell lines. In this study, our main objective was to investigate the comparative effects of NM on anticancer parameters such as cytotoxicity, MMP secretion, and Matrigel invasion in the human uterine sarcoma drug-resistant MES-SA/Dx5 and drug sensitive drug-sensitive MES-SA cell lines. In addition we studied the effect of NM on P-glycoprotein (Pgp) on these cell lines.

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