A Novel Nutrient Mixture Induces Apoptosis in Human Ovarian and Cervical Cancer Cells

M. Waheed Roomi, Bilwa Bhanap, Aleksandra Niedzwiecki*, Matthias Rath

Dr. Rath Research Institute, Oncology Department, Santa Clara, California, USA
Journal of Cervical Cancer Research, 2018, 2(1), 10-17

Abstract: Cervical cancer and ovarian cancer are the deadliest gynecological malignancies and are the fourth and fifth leading causes of death in women respectively. The 5-year survival rate of patients has dropped to 15-20% when these cancers metastasize to  distant  organs.  A  unique  formulation  of  nutrients  containing  green  tea  extract,  ascorbic  acid,  lysine  and  proline,  has exhibited  anticancer  effects  in  various  cancers.  In  our  earlier in  vivo  studies,  the  nutrient  mixture  significantly,  reduced tumor weight and tumor burden of ovarian and cervical cancers.

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A Specific Mixture Of Nutrients Suppresses Ovarian Cancer A-2780 Tumor Incidence, Growth, And Metastasis To Lungs

M. Waheed Roomi, Tatiana Kalinovsky, Matthias Rath, Aleksandra Niedzwiecki
Dr. Rath Research Institute, Santa Clara, California, USA
Nutrients  9:303, 2017

Ovarian cancer is the deadliest gynecological malignancy in women, and fifth leading cause of death. Despite advances made in chemotherapy and surgery, the average time of clinical remission is approximately 2 years and the 5-year survival rate is 45%. Thus, there is an urgent need for the development of a novel therapeutic approach to ovarian cancer treatment. We investigated the effect of a specific nutrient mixture (EPQ) containing ascorbic acid, lysine, proline, green tea extract, and quercetin on human ovarian cancer cell A-2780 in vivo and in vitro.

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Effect of a nutrient mixture on the localization of extracellular matrix proteins in HeLa human cervical cancer xenografts in female nude mice

M.W. Roomi, T. Kalinovsky,  A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
International Journal of Oncology 2015 DOI: 10.3892/ijo.2015.3008


Colorectal, pancreatic and hepatic carcinomas are characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the ECM and basement membrane, allowing cancer cells to spread to distal organs. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of uPA and MMPs with cancer progression, metastasis and shortened patient survival.

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A Nutrient Mixture Modulates Ovarian ES-2 Cancer Progression By Inhibiting Xenograft Tumor Growth And Cellular MMP Secretion, Migration And Invasion

M.W. Roomi, T. Kalinovsky, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
International Journal of Clinical and Experimental Medicine 9(2):814-822, 2016


Epithelial ovarian carcinoma, which occurs mainly in post-menopausal women, is the leading cause of death from gynecological malignancy and the fifth most common cancer in the U.S. Since ovarian cancer often remains clinically silent, the majority of patients with ovarian carcinoma have advanced intraperitoneal metastatic disease at diagnosis, resulting in a poor prognosis. Long-term survival of patients with ovarian cancer remains poor, due to metastasis and recurrence.

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In Vitro Modulation of MMP-2 and MMP-9 in Human Cervical and Ovarian Cancer Cell Lines by Cytokines, Inducers, and Inhibitors

M.W. Roomi, J.C. Monterrey, T. Kalinovsky, M. Rath, A. Niedzwiecki
Oncology Reports 2010, 23: 605-614

Matrix  metalloproteinases (MMPs) secreted by cervical and ovarian cancer, especially  MMP-2 and MMP-9, play crucial roles in tumor invasion and metastasis. We examined the effect of cytokines, mitogens, inducers and inhibitors on MMP-2 and MMP-9 expression in cervical and ovarian cancer cell lines. Human cervical (Hela and DoTc2-4510) and ovarian (SK-OV-3) cell lines were cultured in appropriate media. At near confluence, the cells were washed with PBS and incubated in serum-free medium with various concentrations of several cytokines, mitogens and inhibitors. After 24h the media were removed and analyzed for MMP-2 and MMP-9 by gelatinase zymography and quantitated by densitometry.

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