M. Waheed Roomi, Bilwa Bhanap, Aleksandra Niedzwiecki*, Matthias Rath
Dr. Rath Research Institute, Oncology Department, Santa Clara, California, USA
Journal of Cervical Cancer Research, 2018, 2(1), 10-17
Abstract: Cervical cancer and ovarian cancer are the deadliest gynecological malignancies and are the fourth and fifth leading causes of death in women respectively. The 5-year survival rate of patients has dropped to 15-20% when these cancers metastasize to distant organs. A unique formulation of nutrients containing green tea extract, ascorbic acid, lysine and proline, has exhibited anticancer effects in various cancers. In our earlier in vivo studies, the nutrient mixture significantly, reduced tumor weight and tumor burden of ovarian and cervical cancers.
Based on those results, we studied weather, the antitumor effects are due to induction of apoptosis in these cancers. Cervical cancer cell like (HeLa) and ovarian cancer cells line (SK-OV-3) were cultured in DME media and cells were treated with the nutrient mixture at 0-1000 μg/ml doses. Cell proliferation was measured by MTT assay, morphology was studied by H&E and apoptosis was studied by Live Green Caspase analysis. At 100 μg/ml, the nutrient mixture showed some toxicity to HeLa cells, which considerably increased at 500 μg/ml. However, the nutrient mixture did not affect SK-OV-3 cell growth. H&E staining showed a very similar apoptotic pattern for both the cell lines. HeLa cells and SK-OV-3 cells treated with the nutrient mixture exhibited several characteristics of apoptosis such as shrinkage of cytoplasm, and densely staining nuclei. Both early and late apoptosis were detected moderately at 100 μg/ml, and significant at 1000 μg/ml. These results suggest that the nutrient mixture has therapeutic potential in the treatment of cervical and ovarian cancer by inducing apoptosis of cells
Keywords: Cervical cancer, Ovarian cancer, HeLa, SK-OV-3, Apoptosis, Caspase