A Novel Nutrient Mixture Induces Apoptosis in Human Ovarian and Cervical Cancer Cells

M. Waheed Roomi, Bilwa Bhanap, Aleksandra Niedzwiecki*, Matthias Rath

Dr. Rath Research Institute, Oncology Department, Santa Clara, California, USA
Journal of Cervical Cancer Research, 2018, 2(1), 10-17

Abstract: Cervical cancer and ovarian cancer are the deadliest gynecological malignancies and are the fourth and fifth leading causes of death in women respectively. The 5-year survival rate of patients has dropped to 15-20% when these cancers metastasize to  distant  organs.  A  unique  formulation  of  nutrients  containing  green  tea  extract,  ascorbic  acid,  lysine  and  proline,  has exhibited  anticancer  effects  in  various  cancers.  In  our  earlier in  vivo  studies,  the  nutrient  mixture  significantly,  reduced tumor weight and tumor burden of ovarian and cervical cancers.

Based on those results, we studied weather, the antitumor effects  are  due  to  induction  of  apoptosis  in  these  cancers.  Cervical  cancer  cell  like  (HeLa)  and  ovarian  cancer  cells  line  (SK-OV-3)  were  cultured  in  DME  media  and  cells  were  treated  with  the  nutrient  mixture  at  0-1000  μg/ml  doses.  Cell proliferation  was  measured  by  MTT  assay,  morphology  was  studied  by  H&E  and  apoptosis  was  studied  by  Live  Green Caspase  analysis.  At  100  μg/ml,  the  nutrient  mixture  showed  some  toxicity  to  HeLa  cells, which considerably increased at  500  μg/ml.  However,  the  nutrient  mixture  did  not  affect  SK-OV-3  cell  growth.  H&E  staining  showed  a  very  similar  apoptotic pattern for both the cell lines. HeLa cells and SK-OV-3 cells treated with the nutrient mixture exhibited several characteristics  of  apoptosis  such  as  shrinkage  of  cytoplasm,  and  densely  staining  nuclei.  Both  early  and  late  apoptosis were detected moderately at 100 μg/ml, and significant at 1000 μg/ml. These results suggest that the nutrient mixture has therapeutic potential in the treatment of cervical and ovarian cancer by inducing apoptosis of cells

Keywords: Cervical cancer, Ovarian cancer, HeLa, SK-OV-3, Apoptosis, Caspase