Induction of Apoptosis in Human Lung Cancer Cells (A-549) by a Novel Nutrient Mixture via Upregulation of Caspase Enzymes

Furthermore, our studies also showed that prior supplementation of nutrient mixture was able to reduce urathane induced lung cancer in male A/J mice. Based on the observation, we questioned whether cell apoptosis was partially responsible for this phenomenon. The lung cancer cells A-549 were cultured in complete Ham F-12K medium and the cells were treated with NM at 0-1000 μg/ml concentration. Cell cytotoxicity was measured by MTT assay, morphology by H&E staining, and the apoptosis by Green Caspases. The nutrient mixture did not show significant inhibition of cell proliferation. However, H&E staining at the dose of 100 μg/ml showed a few cellular changes, while significant changes pertaining to apoptosis morphology were observed at 500 and 1000 μg/ml. Live Green Caspases analysis showed cells in early and late apoptosis with increasing doses of NM. Our results suggest that NM may be a new supplemental strategy and deserves further investigation as a potential therapeutic agent in lung cancer.

Key words: Lung cancer; A-549 cells; Apoptosis; Cytotoxicity; MTT; Live green caspase



Ahmed T