In vitro anticarcinogenic effect of a nutrient mixture on human rhabdomyosarcoma cells

MMP secretion was studied by zymography, viability by MTT assay, cell invasion through Matrigel, and morphology and apoptosis by H&E staining and live green caspase kit. Zymography demonstrated MMP-2 secretion and PMA-induced MMP-9 secretion. NM inhibited the secretion of both MMPs in a dose-dependent fashion, with virtual total inhibition at 500 μg/ml NM. Cell invasion through Matrigel was inhibited at 10, 50, 100 and 500 μg/ml by 75%, 80%, 92% and 100% (p = 0.02) respectively. NM was slightly toxic at 1000 μg/ml (20% over control, p = 0.016) to rhabdomyosarcoma cells. Cells exposed to NM showed dose-dependent apoptosis with 90% of cells in late apoptosis at 1000 μg/ml. These results suggest that NM has potential in the treatment of rhabdomyosarcoma by inducing cell apoptosis and inhibiting cell invasion and MMP secretion without toxic effects.

Key Words:
Rhabdomyosarcoma, apoptosis, MMPs, Matrigel invasion, nutrients, green tea extract, ascorbic acid, lysine

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