Cells were also treated with PMA 200 ng/mL to study enhanced secretion of MMP-9. Cell proliferation / cytotoxicity was evaluated by MTT assay, MMP activity by gelatinase zymography, and invasion through Matrigel. The nutrient mixture showed no significant effect on testis cancer cell growth. Zymography demonstrated secretion of MMP-2 by untreated human testis cancer cells and MMP-9 with PMA induction. NM inhibited secretion of both MMPs in a dose-dependent fashion with virtual total inhibition of MMP-9 at 100 μg/mL. Invasion of human testis cancer cells through Matrigel was reduced by 84% at 50 μg/mL and at 100 μg/mL (p = 0.004). NM significantly inhibited MMP secretion and matrix invasion in testicular cancer cells without toxic effect, indicating potential as an anticancer agent.
Testicular cancer, MMPs, Matrigel invasion, nutrients, green tea extract, ascorbic acid, lysine