Inhibition of growth and expression of inflammation mediators in human leukemic cell line u-937 by a nutrient mixture

M.W. Roomi, T. Kalinovsky, N.W. Roomi, M. Rath and A. Niedzwiecki
Dr. Rath Research Institute, Cancer Division, Santa Clara, CA, USA
Experimental Oncology 2013; 35(3): 180-186

Abstract:
A nutrient mixture (NM) containing ascorbic acid, lysine, proline and green tea extract has exhibited anticancer activity in vitro and in vivo in a number of cancer cell lines. We investigated the effect of NM on human leukemic myeloid U-937 cells in vitro by measuring: cell proliferation, MMP expression, invasion, apoptosis, and COX-2 and COX-1 protein expression. Human leukemic cell line U-937 (ATCC) was cultured in RPMI medium supplemented with fetal bovine serum and antibiotics.

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In vitro and in vivo inhibition of human Fanconi anemia head and neck squamous carcinoma by a novel nutrient mixture

M.W. Roomi, T. Kalinovsky, N.W. Roomi, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
International Journal of Oncology 2012; 41(6), 1996-2004

Abstract
Head and neck squamous cell carcinoma (HNSCC) and acute myeloid leukemia are the major causes of mortality and morbidity in Fanconi anemia (FA) patients. The objective of this study was to investigate the antineoplastic activity of NM, a novel antineoplastic nutrient mixture (containing lysine, proline, ascorbic acid and green tea extract) on human FA HNSCC in vitro and in vivo. Human FA HNSCC cell line OHSU-974 (Fanconi Anemia Research Fund) was cultured in RPMI medium supplemented with 20% FBS and antibiotics. At near confluence, cells were treated in triplicate with different concentrations of NM: 0, 50, 100, 250, 500 and 1000 µg/ml.

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In vivo and in vitro antitumor effects of nutrient mixture in murine leukemia cell line P-388

Roomi MW, Roomi NW, Bhanap B, Rath M, Niedzwiecki A                                                        

ExpOncol. 2011; 33(2):71-7

Abstract:

Aim: Leukemia is characterized by uncontrolled marrow cell proliferation and metastatic foci. We investigated the antitumor potential of a nutrient mixture on malignant leukemia P-388 cells. Methods: The nutrient mixture containing lysine, proline, ascorbic acid, green tea extract and other nutrients is formulated to target key pathways in cancer progression.

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Nutrient Mixture Inhibits in vitro and in vivo Growth of Human Acute Promyelocytic Leukemia HL-60 Cells

MW Roomi, NW Roomi, B Bhanap, A Niedzwiecki, M Rath;                                                        

Exp Oncol. 2011;33(4):212-215

Abstract:

Aim:Untreated acute promyelocytic leukemia is the most malignant form of acute leukemias, with median survival of less than one month. We investigated in vitro and in vivo synergistic effects of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract, on acute promyelocytic leukemia HL-60 cells.

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Antineoplastic effects of nutrient mixture on raji and jurkat T cells: the two highly aggressive Non-Hodgkin's Lymphoma cell lines

Roomi MW, Bhananp BA, Roomi NW, Rath M, Niedzwiecki A
Experimental Oncology 2009; 31(3): 149-155

Abstract:
Non-Hodgkin lymphomas incidence has increased more than 70% in last 25 years. Aggressiveness, higher relapse rate, and treatment complications pose significant barriers. Decreased food intake and side effects of treatments make cancer patients vulnerable to deficiency of essential nutrients such as vitamin C, lysine, and proline leading to the formation of weak extra cellular matrix susceptible to easy breakdown by matrix metalloproteinase enzymes.

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