In Vitro Effect of Cytokines, Inducers, and Inhibitors on the Secretion of MMP-2 and MMP-9 in Hepatocarcinoma Cell Line SK-Hep-1

Roomi MW, Kalinovsky T, Bhanap B, Niedzwiecki A, Rath M

Integrative Cancer Therapies Volume 18: 1–12, 2019 DOI: 10.1177/1534735419889155

 

Abstract:

The prognosis of hepatocellular carcinoma (HCC) remains dismal despite any treatment. Matrix metalloproteinases (MMPs) have been researched for their role in tumor invasion and metastasis. Various cytokines, mitogens, growth factors, inducers, and inhibitors control MMP activities. In this article, we investigated the roles of these in the regulation of MMP2, -9 secretions in HCC. Human HCC SK-Hep-1 was grown in appropriate media. At near confluence, the cells were washed with phosphate-buffered saline and incubated in serum-free media with PMA; TNF-α, IL-1β; lipopolysaccharide; epigallocatechin gallate (EGCG) and doxycycline (Dox) at various doses with and without PMA; a nutrient mixture (NM) containing lysine, proline, ascorbic acid, and EGCG with and without PMA at; and actinomycin D and cycloheximide at different doses. After 24 hours, the media were removed and analyzed. SK-Hep-1 expressed bands corresponding to MMP-2 and MMP-9. TNF-α showed an insignificant effect on MMP-2 at doses below 25 at which dose MMP-2 was virtually blocked and a moderate dose-dependent effect on MMP-9.

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Antitumor effect of a nutrient mixture on colon cancer cells

M. W. Roomi, B. Bhanap, A. Niedzwiecki, M. Rath

Journal of Cellular Medicine and Natural Health, July 2019

 

Abstract:

Colorectal cancer is the second most deadly cancer in the United States and there is an urgent need to develop effective preventive and therapeutic strategies against it. The aim of our study was to investigate the antitumor effects of a nutrient mixture containing ascorbic acid, lysine, proline and green tea extract that has been previously demonstrated a strong anticancer activity in several cancer cell types. We studied the effect of NM on human colon cancer cell line HCA-7 in vitro by measuring cancer cell proliferation, invasion, and expression of pro-inflammatory COX-2 and COX-1 proteins.

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Modulation Of Upa, Mmps And Their Inhibitors By A Novel Nutrient Mixture In Human Colorectal, Pancreatic And Hepatic Carcinoma Cell Lines

Roomi MW, Kalinovsky T, Niedzwiecki A, Rath M

Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
International Journal of Oncology –2015; 47(1): 370-376

Abstract:

Colorectal, pancreatic and hepatic carcinomas are characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the ECM and basement membrane, allowing cancer cells to spread to distal organs. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of uPA and MMPs with cancer progression, metastasis and shortened patient survival.

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Micronutrient Synergy in the Fight Against Hepatocellular Carcinoma

M. W. Roomi, N. W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050, USA
Cancers 2012, 4(2):323-339

Abstract: 
The incidence of hepatocellular carcinoma (HCC), once thought to be a rare tumor in North America, has rapidly increased in recent years in the United States. Current treatment modalities to halt the progression of this disease are only marginally effective. The mainstay treatment is liver transplantation, which is often confronted with donor shortage. Invasion, metastasis and recurrence contribute to the high mortality rate of this disease. Matrix metalloproteinases (MMPs) that degrade the extracellular matrix (ECM) have been associated with the progression, invasion and metastasis of the disease.

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In Vivo and In Vitro Effect of a Nutrient Mixture of Chemopreventive Agents on Human Hepatocarcinoma Cells

M.W. Roomi, N.W. Roomi, T. Kalinovsky, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050

Exp Oncol 2010, 32: 84-91

Abstract

Purpose: Long-term survival of patients with hepatocellular carcinoma (HCC), a common cancer worldwide, remains poor, due to metastasis and recurrence. We investigated the effect of a novel nutrient mixture (NM) of chemopreventive agents containing ascorbic acid, lysine, proline, and green tea extract on human HCC cell line Sk-Hep-1 in vivo and in vitro.

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