M.W. Roomi, T. Kalinovsky, N.W. Roomi, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Oncology Reports 2013; 29: 1714-1720

Abstract
Neuroblastoma, a peripheral nervous system cancer that can be highly invasive and metastatic, accounts for 8-10% of all solid childhood tumors in children under the age of 15 years. Despite multiple clinical efforts, prognosis remains poor in this enigmatic disease. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant antitumor effects.  Using the nude mouse xenograft model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating viability, MMP-2 and -9 secretion, TIMP-2 secretion, Matrigel invasion and apoptosis and morphology.  Athymic nude mice 5-6 weeks of age were inoculated with 3x106 neuroblastoma cells SK-N-MC subcutaneously and randomly divided into two groups.

M W Roomi, N W. Roomi, B Bhanap, A Niedzwiecki, M Rath

OJApo 2013; 2(4); 37-44

Abstract:

A majority of oral cancers is squamous cell carcinoma and tongue carcinomas comprise 30% of all oral cancers. Phytochemicals, herbal and dietary antioxidants have been reported to prevent cancers. A nutrient mixture containing, ascorbic acid, lysine, proline and green tea extract, among other nutrients, has previously been shown to exhibit a broad spectrum chemo preventative and therapeutic anti-cancer properties in a number of cell lines. In a recent study, we found that the nutrient mixture significantly inhibited acetaminophen induced hepatic and renal toxicity, and it suppressed carbon tetrachloride induced hepatic toxicity in ICR mice as well.

M.W. Roomi, N.W. Roomi, T. Kalinovsky, M. Rath, A. Niedzwiecki
Integrative Cancer Therapies 2009; 8(2): 168-176

Head and neck squamous cell carcinomas (HNSCC) are known for their aggressive growth and propensity to metastasize. We investigated the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HNSCC cell line FaDu in vivo and in vitro. Athymic male nude mice (n=12) were inoculated with 3 x 106 FaDu cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighted and processed for histology.

M.W. Roomi, T. Kalinovsky, M. Rath, A. Niedzwiecki

Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Head and Neck Cancer, Avid Science 2017, pp 2-23

Head and neck squamous cell carcinoma (HNSCC), which develops in the mucous membranes of the oropharynx, nasopharynx, larynx and hypopharynx, can metastasize to other parts of the body, such as the lymph nodes or lungs leading to poor prognosis. Matrix metalloproteinases (MMPs), especially MMP-2 and -9, play critical roles in degradation of the extracellular matrix and their expression in head and neck carcinoma have been associated with progression and invasion of these tumors. In our studies we targeted various mechanisms of cancer by designing specific combinations of different polyphenols (PB) and combinations of polyphenols with vitamins and other micronutrients (NM).