Modulation of MMP-2 and MMP-9 secretion by cytokines, inducers and inhibitors in human glioblastoma T-98G cells

M. Waheed Roomi, Tatiana Kalinovsky, Matthias Rath, Aleksandra Niedzwiecki

Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
ONCOLOGY REPORTS 37: 1907-1913, 2017

Abstract:

Brain tumors are highly aggressive, characterized by the secretion of high levels of matrix metalloproteinase (MMP)-2 and MMP-9 that degrade the extracellular matrix and basement membrane, allowing cancer cells to spread to distal organs. Various cytokines, mitogens, growth factors, inducers and inhibitors control MMP activity. We investigated the roles of these in the regulation of MMP-2 and MMP-9 in human glioblastoma T-98G cells.

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Inhibitory effects of a novel nutrient mixture on MMP secretion and invasion on human thyroid cancer cell line SW 579

M.W. Roomi, B. Bhanap, A. Niedzwiecki, and M. Rath
JANA 2009, 13(1): 26-34

Abstract: 
Thyroid cancer is the most common endocrine malignancy. Mortality from thyroid cancer results from tumor invasion with local and distant metastasis. Degradation of extracellular matrix is the hallmark of metastasis and is mediated by matrix metalloproteinase (MMP) enzymes. A novel nutrient mixture (NM) containing ascorbic acid, lysine, proline and green tea extract has exhibited significant anticancer activity in other cancer cell lines.

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Inhibition of Glioma Cell Line A-172 MMP Activity and Cell Invasion In Vitro by a Nutrient Mixture

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2007, 24(2): 231-238

Standard multimodality therapy of gliomas is associated with poor patient survival and significant toxicity. Abnormal expression of matrix metalloproteinases (MMPS) is associated with tumor growth and invasion. We investigated the effect of a combination of natural compounds (NM), primarily composed of lysine, proline, ascorbic acid and green tea extract in vitro on glioma cell line A-172, by measuring MMP secretion, invasion through Matrigel, and cell proliferation. Glioma cells A-172 (ATCC) were grown in modified Dulbecco’s Eagle medium with10% fetal bovine serum and antibiotics and treated with NM at 0, 10, 50, 100, 500 and 1000 µg/ml concentration in triplicate at each dose. Cell proliferation was assayed by MTT, MMP secretion by zymography, invasion through Matrigel, and morphology by H&E staining.

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