Suppression of metastasis of intratesticular inoculation of B16FO melanoma cells by a novel nutrient mixture in male athymic nude mice

M.W. Roomi, T. Kalinovsky, N.W. Roomi,  A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental and Therapeutic Medicine 2012; 4: 775-780

Metastasis, commonly to the lung, is the major cause of death from testicular cancer. We studied the effect of a novel nutrient mixture (NM) containing ascorbic acid, amino acids and green tea extract on inhibition of melanoma growth and metastasis using a model of intratesticular inoculation of B16FO cells into nude mice. Male athymic mice (n=12), 10-12 weeks of age, were inoculated with 5X105 B16FO melanoma cells in 100 µL of PBS into the right testis; the left testis was left untreated. After inoculation, the mice were randomly divided into two groups. The Control group (n=6) was fed a regular mouse chow diet and the NM 1% group (n=6) the same diet but supplemented with 1% NM. Four weeks later the mice were sacrificed and the abdominal cavity was opened.


Mice in the Control group exhibited extensive metastasis in the peritoneal cavity and severely enlarged right testes and necrotic seminiferous tubules. In contrast, in the NM 1% fed group there was no evidence of peritoneal metastasis in 50% of the animals and mild metastasis in the other 50%.The right testes were enlarged and seminiferous tubules in the area of invasion showed evidence of degeneration. In both groups, there was no metastasis to liver, kidney and spleen. However, severe lung metastasis was observed in 2 of 6 mice in the Control group and mild metastasis in 2 of 6 mice in the NM 1% group.  In conclusion, these results corroborate with our earlier studies and confirm anti-metastatic potential of NM.

Key words: 
metastasis, intratesticular injection, melanoma B16FO cells, athymic nude mice, nutrient mixture