M.W. Roomi, V.Ivanov, T.Kalinovsky, A. Niedzwiecki, M.Rath
Journal of the American Nutraceutical Association 2004, 7(2): 40-43
Limitation of current treatment methodologies to control metastasis, as well as the proposed antitumor properties of specific nutrients prompted us to investigate the effect of a specific formulation (NS) of lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on human colon cancer cells HCT 116 for viability, MMP expression, invasion, and morphology.
Cell proliferation was based on MTT assay and MMP expression in condition media by gelatinase zymography. Invasion through Matrigel™ was evaluated and morphology was assessed by Hematoxylin and Eosin staining. NS did not demonstrate antiproliferative effect up to 1000 μg/ml concentration. Cancer cell invasion through Matrigel was significantly reduced at 100 μg/ml (76%, p=0.0008) and completely inhibited at 500 μg/ml (p=0.0002). Gelatinase zymography showed dose dependent inhibition of MMP-9 expression by NS with virtual total inhibition at 100 μg/ml. Our results suggest that the specific formulation of nutrients tested is an excellent candidate for therapeutic use in the treatment of colon cancer, by inhibiting MMP expression and invasion.
Colon cancer, MMP, nutrient synergy, anti-tumor effect