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Induction of Apoptosis in Human Lung Cancer Cells (A-549) by a Novel Nutrient Mixture via Upregulation of Caspase Enzymes

M.W. Roomi, Ph.D., Ahmed T, A. Niedzwiecki, Ph.D., M. Rath, M.D.

Dr. Rath Research Institute,  1260 Memorex Drive, Santa Clara, CA 95050

Austin J Lung Cancer Res. 2018; 3(1): 1013 – 1016


Lung cancer is the leading cause of death in western world, and the incidence continues to increase. Lung cancer is frequently diagnosed when it is extensively metastasized and has a dismal prognosis. Chemotherapy being the mainstay of treatment has not been able to increase quality of life or survival of patients. A novel nutrient mixture containing green tea extract, ascorbic acid, lysine, and proline exhibited anti-cancer effects in various cancers. In our earlier studies, the nutrient mixture considerably reduced the tumor weight and tumor burden in athymic mice using A-549 lung cancer cells.
Furthermore, our studies also showed that prior supplementation of nutrient mixture was able to reduce urathane induced lung cancer in male A/J mice. Based on the observation, we questioned whether cell apoptosis was partially responsible for this phenomenon. The lung cancer cells A-549 were cultured in complete Ham F-12K medium and the cells were treated with NM at 0-1000 μg/ml concentration. Cell cytotoxicity was measured by MTT assay, morphology by H&E staining, and the apoptosis by Green Caspases. The nutrient mixture did not show significant inhibition of cell proliferation. However, H&E staining at the dose of 100 μg/ml showed a few cellular changes, while significant changes pertaining to apoptosis morphology were observed at 500 and 1000 μg/ml. Live Green Caspases analysis showed cells in early and late apoptosis with increasing doses of NM. Our results suggest that NM may be a new supplemental strategy and deserves further investigation as a potential therapeutic agent in lung cancer.

Key words: Lung cancer; A-549 cells; Apoptosis; Cytotoxicity; MTT; Live green caspase



Ahmed T