M.W. Roomi, T. Kalinovsky, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental Oncology 38(1):1-3, 2016
Brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and ECM components. We previously demonstrated the effectiveness of a nutrient mixture containing ascorbic acid, lysine, proline, and green tea extract in vitro: on activity of urokinase plasminogen activator, matrix metalloproteinases and TIMPs in various human glioblastoma (LN-18, T-98G and A-172) cell lines and on glioblastoma A-172 cell proliferation and Matrigel invasion.
Our main objective in this study was to investigate the effect of the nutrient mixture in vivo on human glioblastoma U-87MG cell line. Athymic male nude mice (n=12) were inoculated with 3x106 U-87-MG cells subcutaneously and randomly divided into two groups: group A was fed a regular diet and group B a regular diet supplemented with 0.5% of the nutrient mixture. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. Dimensions (length and width) of tumors were measured using a digital caliper, and the tumor burden was calculated using the following formula: 0.5 x length x width. The nutrient mixture inhibited tumor weight and burden of U-87MG tumors by 53% (p = 0.015) and 48% (p=0.010), respectively. These results suggest that NM has therapeutic potential in treatment of glioblastoma.
Running title: Nutrients exhibit different responses in FA fibroblasts than NHDF
Key words: glioblastoma U-87 MG cell line, nutrients, xenograft tumor growth