We investigated the effect of NM on MM cell line MSTO-211 H proliferation (by MTT assay), MMP activity (by gelatinase zymography), invasion (through Matrigel) and morphology (by H&E staining).
MMP-2 and PMA-induced MMP-9 secretion were inhibited by NM in a dose-dependent fashion with virtual total inhibition at 500 mg/ml NM. Invasion through Matrigel was inhibited at 50, 100 and 500 mg/ml by 27, 36 and 100% respectively. NM was not toxic to the MM cell line, and H&E staining did not indicate any changes even at the highest concentration.
In conclusion, NM significantly inhibited MM cell MMP secretion and invasion- both important parameters for cancer prevention, suggesting NM as an effective treatment strategy for MM.