John Cha*, M. Waheed Roomi*, Tatiana Kalinovsky, Aleksandra Niedzwiecki, Matthias Rath
Dr. Rath Research Institute, Santa Clara, California, USA
International Journal of Oncology 2016, 49(3), 895-902
Cancer progression is characterized by loss of extracellular matrix (ECM) integrity, which is a precondition for tumor growth and metastasis. In order to elucidate the precise mechanisms of ECM degradation in cancer we used a genetically modified mouse mimicking two distinct human metabolic features associated with cancerogenesis - the lack of endogenous vitamin C synthesis and the production of human Lp(a). Female Lp(a)+;Gulo(-/-) and control wild type Balb/c mice without these two metabolic features were orthotopically inoculated with 4T1 breast cancer cells (5x105).