Downregulation of urokinase plasminogen activator and matrix metalloproteinases and upregulation of their inhibitors by a novel nutrient mixture in human prostate cancer cell lines PC-3 and DU-145

M.W. Roomi, T. Kalinovsky, M. Rath and A. Niedzwiecki
Dr. Rath Research Institute, Cancer Division, Santa Clara, CA, USA
Oncology Reports 2011  DOI: 10.3892/or.2011.1434

Strong clinical and experimental evidence shows that elevated levels of u-PA and MMPs are associated with prostate cancer progression, metastasis and shortened survival in patients. MMP activities are regulated by specific tissue inhibitors of metalloproteinases (TIMPs). A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract showed anticancer activity against a number of cancer cell lines. Our main objective was to study the effect of NM on activity of u-PA, MMPs and their inhibitor TIMPs on human prostate cancer cell lines PC-3 and DU-145. Human prostate cancer cell lines PC-3 and DU-145 (ATCC) were grown in MEM media with 10% FBS and antibiotics in 24-well tissue culture plates. At near confluence, the cells were treated with NM at 0-1000 μg/ml in triplicate at each concentration.

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In Vivo Antitumor Effect of Ascorbic Acid, Lysine, Proline, and Green Tea Extract on Human Prostate Cancer PC-3 Xenografts in Nude Mice: Evaluation of Tumor Growth and Immunohistochemistry

M.W. Roomi, V.Ivanov, T.Kalinovsky, A. Niedzwiecki, M.Rath
In Vivo 2005, 19(1): 179-184.

Prostate cancer, a highly metastatic cancer, is the second most deadly cancer in the United States. Cancer cell invasion through the extracellular matrix (ECM), and subsequent metastasis is dependent upon degradation of the ECM. Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), Ki 67 (proliferative protein), and constituents of ECM play a critical role in angiogenesis, and are crucial in neoplastic invasion and metastasis.

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Antitumor Effect of Ascorbic Acid, Lysine, Proline, Arginine and Epigallocatechin Gallate in Prostate Cancer Cell Lines PC-3, LNCaP, and DU145

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Research Communications in Molecular Pathology and Pharmacology 2004, 115-116: 251-64.

Once prostate cancer has metastasized, current treatment methods are generally ineffective. Due to the reported anti-tumor properties of specific nutrients, we investigated the effect of a unique formulation (NS) of lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on human prostate cancer cell lines: PC-3, DU145 (androgen insensitive) and LNCaP (androgen sensitive), by measuring cell proliferation, MMP expression, and invasion potential.

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