M.W. Roomi, T. Kalinovsky, N.W. Roomi, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental and Therapeutic Medicine 2012; 4: 775-780
Metastasis, commonly to the lung, is the major cause of death from testicular cancer. We studied the effect of a novel nutrient mixture (NM) containing ascorbic acid, amino acids and green tea extract on inhibition of melanoma growth and metastasis using a model of intratesticular inoculation of B16FO cells into nude mice. Male athymic mice (n=12), 10-12 weeks of age, were inoculated with 5X105 B16FO melanoma cells in 100 µL of PBS into the right testis; the left testis was left untreated. After inoculation, the mice were randomly divided into two groups. The Control group (n=6) was fed a regular mouse chow diet and the NM 1% group (n=6) the same diet but supplemented with 1% NM. Four weeks later the mice were sacrificed and the abdominal cavity was opened.