Cancer

A novel nutrient mixture inhibits chondrosarcoma invasion and metastasis parameters

M. W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath
Dr. Rath Research Institute, Santa Clara, CA
Horizons in Cancer Research, volume 59, Nova Science Publishers, Inc., 2015

Abstract:

Chondrosarcoma (CS), a malignancy of cartilaginous origin, is an aggressive cancer with poor prognosis, due to both its aggressive metastatic spread and the lack of efficacy in current treatment modalities to prevent or counteract tumor progression. Chondrosarcomas are characterized by high levels of proteases, which are involved in the degradation of ECM and the basement membrane, thus allowing cancer cells to invade and metastasize to distal organs. 

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Naturally Produced Extracellular Matrix Inhibits Growth Rate and Invasiveness of Human Osteosarcoma Cancer Cells

V. Ivanov, S. Ivanova, M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2007, 24(2): 209-217

Abstract:
Enhanced supplementation with certain naturally occuring nutrients and vitamins has been associated with a reduction in occurence and progression of human cancer. The exact mechanisms of this action are still under investigation. Extracellular matrix (ECM) plays a key role in the development of cancer. Therefore, we studied whether nutrients could exert anticancer effects through alteration of ECM biological properties.

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Effect of Ascorbic Acid, Lysine, Proline and Green Tea Extract on Human Osteosarcoma Cell Line MNNG-HOS Xenografts in Nude Mice: Evaluation of Tumor Growth and Immunohistochemistry

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2006, 23(3): 411-417

Introduction:
Structural changes in the extracellular matrix (ECM) are necessary for cell migration during tissue remodeling. MMPs, VEGF, Ki 67 (proliferative protein), and constituents of ECM play a critical role in angiogenesis and underlie neoplastic invasion and metastasis.

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Antitumor Effect of Nutrient Synergy on Human Osteosarcoma Cells U2OS, MNNG-HOS, and Ewing’s Sarcoma SK-ES.1

M.W. Roomi, V.Ivanov, T.Kalinovsky, A. Niedzwiecki, M.Rath
Oncology Reports 2005, 13(2): 253-257

Abstract:
Current treatment of osteosarcoma is associated with poor prognosis, especially due to the increased risk of developing other cancers with chemotherapy. Therefore, new safe effective treatment strategies are needed. We investigated the effect of a unique mixture of nutrients containing: lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate (EGCG) on human osteosarcoma cell lines: U-2OS, MNNG-HOS, and Ewing SK-ES-1 by measuring: cell proliferation, expression of MMP-2, MMP-9, and invasive and angiogenesis potential.

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In Vivo and In Vitro Antitumor Effect of Nutrient Synergy on Human Osteosarcoma Cell Line MNNG-HOS

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Annals of Cancer Research and Therapy 2004, 12(1-2): 135-145

Abstract:
Current treatment of osteosarcoma is associated with poor prognosis, especially due to the increased risk of developing other cancers with chemotherapy. Therefore, new safe effective treatment strategies are needed. This prompted us to investigate the synergistic effect of a nutrient mixture (NS) of lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on the growth of human osteosarcoma xenografts in athymic nude mice. We also investigated the effect of NS on human osteosarcoma cell line MNNG-HOS in vitro by measuring: cytotoxicity, modulation of MMP-2 and -9, cancer cell invasive potential, and angiogenesis.

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