A Nutrient Mixture Inhibits Glioblastoma Xenograft U-87 Mg Growth In Male Nude Mice

M.W. Roomi, T. Kalinovsky, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental Oncology 38(1):1-3, 2016

Abstract: 

Brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and ECM components. We previously demonstrated the effectiveness of a nutrient mixture containing ascorbic acid, lysine, proline, and green tea extract in vitro:  on activity of urokinase plasminogen activator, matrix metalloproteinases and TIMPs in various human glioblastoma (LN-18, T-98G and A-172) cell lines and on glioblastoma A-172 cell proliferation and Matrigel invasion.

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Inhibition of Glioma Cell Line A-172 MMP Activity and Cell Invasion In Vitro by a Nutrient Mixture

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2007, 24(2): 231-238

Standard multimodality therapy of gliomas is associated with poor patient survival and significant toxicity. Abnormal expression of matrix metalloproteinases (MMPS) is associated with tumor growth and invasion. We investigated the effect of a combination of natural compounds (NM), primarily composed of lysine, proline, ascorbic acid and green tea extract in vitro on glioma cell line A-172, by measuring MMP secretion, invasion through Matrigel, and cell proliferation. Glioma cells A-172 (ATCC) were grown in modified Dulbecco’s Eagle medium with10% fetal bovine serum and antibiotics and treated with NM at 0, 10, 50, 100, 500 and 1000 µg/ml concentration in triplicate at each dose. Cell proliferation was assayed by MTT, MMP secretion by zymography, invasion through Matrigel, and morphology by H&E staining.

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Marked inhibition of growth and invasive parameters of head and neck squamos carcinoma fadu by a nutrient mixture

M.W. Roomi, N.W. Roomi, T. Kalinovsky, M. Rath, A. Niedzwiecki
Integrative Cancer Therapies 2009; 8(2): 168-176

Head and neck squamous cell carcinomas (HNSCC) are known for their aggressive growth and propensity to metastasize. We investigated the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HNSCC cell line FaDu in vivo and in vitro. Athymic male nude mice (n=12) were inoculated with 3 x 106 FaDu cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighted and processed for histology.

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Inhibitory effects of a novel nutrient mixture on MMP secretion and invasion on human thyroid cancer cell line SW 579

M.W. Roomi, B. Bhanap, A. Niedzwiecki, and M. Rath
JANA 2009, 13(1): 26-34

Abstract: 
Thyroid cancer is the most common endocrine malignancy. Mortality from thyroid cancer results from tumor invasion with local and distant metastasis. Degradation of extracellular matrix is the hallmark of metastasis and is mediated by matrix metalloproteinase (MMP) enzymes. A novel nutrient mixture (NM) containing ascorbic acid, lysine, proline and green tea extract has exhibited significant anticancer activity in other cancer cell lines.

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Inhibition of human neuroblastoma cell line SK-N-MC in vivo and in vitro by a novel nutrient mixture

M.W. Roomi, T. Kalinovsky, N.W. Roomi, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Oncology Reports 2013; 29: 1714-1720

Abstract
Neuroblastoma, a peripheral nervous system cancer that can be highly invasive and metastatic, accounts for 8-10% of all solid childhood tumors in children under the age of 15 years. Despite multiple clinical efforts, prognosis remains poor in this enigmatic disease. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant antitumor effects.  Using the nude mouse xenograft model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating viability, MMP-2 and -9 secretion, TIMP-2 secretion, Matrigel invasion and apoptosis and morphology.  Athymic nude mice 5-6 weeks of age were inoculated with 3x106 neuroblastoma cells SK-N-MC subcutaneously and randomly divided into two groups.

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More Articles ...

  1. Modulation of u-PA, MMPs and their inhibitors by a novel nutrient mixture in human glioblastoma cell lines
  2. In Vitro and In Vivo Inhibition of Human Fanconi Anemia Head and Neck Squamous Carcinoma by a Phytonutrient Combination
  3. Cytokines, Inducers And Inhibitors Modulate Mmp-2 And Mmp‑9 Secretion By Human Fanconi Anemia Immortalized Fibroblasts

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