In Vivo and In Vitro Antitumor Effect of Ascorbic Acid, Lysine, Proline, Arginine and Green Tea Extract on Human Fibrosarcoma Cells HT-1080

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2006, 23(1): 105-112

Introduction:
Current treatment of fibrosarcoma, an aggressive cancer of the connective tissue, is generally associated with poor prognosis. Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and constituents of the ECM, such as fibronectin, play a critical role in angiogenesis and underlie neoplastic invasion and metastasis. This and anticancer properties of lysine, proline, arginine, ascorbic acid, and green tea extract (NM) prompted us to investigate the effect of these nutrients in vitro on human fibrosarcoma cells HT-1080 by measuring: cell proliferation, modulation of MMP-2 and MMP-9, and invasive potential.

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Synergistic Antitumor Effect of Ascorbic Acid, Lysine, Proline, and Epigallocatechin Gallate on Human Fibrosarcoma Cells HT-1080

M.W. Roomi, V.Ivanov, T.Kalinovsky, A. Niedzwiecki, M.Rath
Annals of Cancer Research and Therapy 2004, 12(1-2): 146-156

Abstract:
Matrix metalloproteinases (MMPs) have received much attention in recent years for their role in various malignancies, and have been implicated in tumor invasion, metastasis and angiogenesis. Certain MMPs, such as MMP-2 and MMP-9, have shown a special propensity for promoting cancer. Development of MMP inhibitors has been a recent approach to controlling cancer and blocking metastasis.

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In Vitro and In Vivo Antitumor Effect of a Nutrient Mixture Containing Ascorbic Acid, Lysine, Proline, and Green Tea Extract on Human Synovial Sarcoma Cancer Cells

 

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Journal of the American Nutraceutical Association 2006, 9(2): 30-34

Introduction:
Synovial sarcoma, a soft tissue cancer that most often occurs around leg or arm joints, has a 50% rate of metastasis. Structural changes in the extracellular matrix (ECM) are necessary for cancer cell migration and invasion.

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In vitro anticarcinogenic effect of a nutrient mixture on human rhabdomyosarcoma cells

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Gene Therapy and Molecular Biology 2007, 11: 133-142

Abstract:
Rhabdomyosarcoma, the most common pediatric soft tissue sarcoma of mesenchymal origin, has metastasized in ~25% of all patients at time of diagnosis. Though current treatment strategies have achieved some success, they are associated with severe adverse effects. We investigated the effect of a nutrient mixture (NM), which has shown antitumor effects on various cancer cell lines, on rhabdomyosarcoma cell growth, apoptosis, MMP secretion, and invasion. Human rhabdomyosarcoma cells, grown in DME, were treated at near confluence with NM at 0, 10, 50, 100, 500 and 1000 μg/ml in triplicate at each dose.

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Inhibition of Cell Invasion and MMP Production by a Nutrient Mixture in Malignant Liposarcoma Cell Line SW-872

M.W. Room, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2007, 24: 394-401

Abstract:
Liposarcoma, a malignancy of fat cells, is the most common soft tissue sarcoma. Though rare, poorly differentiated liposarcomas commonly metastasize to lungs and liver, leading to poor prognosis. Prevention of Extracellular matrix (ECM) degradation by inhibition of matrix metalloproteinases (MMPs) activity has been shown to be a promising therapeutic approach to inhibition of cancer progression. A nutrient mixture (NM) containing lysine, proline, ascorbic acid, and green tea extract has shown significant anticancer activity against a number of cancer cell lines. We investigated the effect of NM on liposarcoma cell line SW-872 proliferation (MTT assay), MMP secretion (gelatinase zymography), invasion through Matrigel, and apoptosis and morphology (live green caspase kit and H&E).

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More Articles ...

  1. Modulation of u-PA, MMPs and their inhibitors by a novel nutrient mixture in adult human sarcoma cell lines
  2. Modulation of u-PA, MMPs and their inhibitors by a novel nutrient mixture in pediatric human sarcoma cell lines
  3. In vitro modulation of MMP-2 and MMP-9 in adult human sarcoma cell lines by cytokines, inducers, and inhibitors

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