Anti-cancer potential of a specific mixture of phytonutrients in bone cancer cells

M.W. Roomi, B. Bhanap, A. Niedzwiecki, M. Rath

Journal of Cellular Medicine and Natural Health, Oct 2018

 

Abstract:

Chondrosarcoma, a malignant tumor of cartilaginous origin, is the most common of the adult primary bone cancers. Forty percent of primary bone cancers are chondrosarcoma. However as chondrosarcoma typically does not respond to radiation and chemotherapy, surgery is the main treatment option. Cancer mortality usually results from the local and distant metastasis. Based on the poor prognosis of chondrosarcoma, we investigated effects of a phytonutrient mixture (PB) containing quercetin, CruciferexTM, curcumin, resveratrol and green tea extract on several parameters associated with the development of this type of cancer.

The chondrosarcoma cells SW-1353 exposed to PB concentrations of 10, 25, 50, and 100 µg/ml were tested for cell proliferation/viability, morphology, matrix metalloproteinase (MMP) secretion, as well as their invasive and migration abilities. Cell proliferation was carried out by MTT assay, MMPs secretion by zymography, cell invasion by Matrigel assay, cellular migration by scratch test and morphology by H&E staining. The PB inhibited cell proliferation in dose-dependent manner starting at 25µg/ml concentration. Zymography demonstrated two bands corresponding to MMP-2 and MMP-9 with enhanced MMP-9 expression upon PMA treatment. The PB inhibited expression of both MMPs in a dose-dependent manner in both PMA-stimulated and non-stimulated cells. Matrigel invasion was totally blocked at 25µg/ml. The H&E staining showed that the PB mixture at 25µg/ml to 100 µg/ml induced apoptotic changes in SW-1353 cells. Significant inhibition of important hallmarks of cancer progression by PB mixture suggests that this nutrient composition should be explored further as a therapeutic agent in chondrosarcoma.

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A novel nutrient mixture inhibits chondrosarcoma invasion and metastasis parameters

M. W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath
Dr. Rath Research Institute, Santa Clara, CA
Horizons in Cancer Research, volume 59, Nova Science Publishers, Inc., 2015

Abstract:

Chondrosarcoma (CS), a malignancy of cartilaginous origin, is an aggressive cancer with poor prognosis, due to both its aggressive metastatic spread and the lack of efficacy in current treatment modalities to prevent or counteract tumor progression. Chondrosarcomas are characterized by high levels of proteases, which are involved in the degradation of ECM and the basement membrane, thus allowing cancer cells to invade and metastasize to distal organs. 

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Naturally Produced Extracellular Matrix Inhibits Growth Rate and Invasiveness of Human Osteosarcoma Cancer Cells

V. Ivanov, S. Ivanova, M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2007, 24(2): 209-217

Abstract:
Enhanced supplementation with certain naturally occuring nutrients and vitamins has been associated with a reduction in occurence and progression of human cancer. The exact mechanisms of this action are still under investigation. Extracellular matrix (ECM) plays a key role in the development of cancer. Therefore, we studied whether nutrients could exert anticancer effects through alteration of ECM biological properties.

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Effect of Ascorbic Acid, Lysine, Proline and Green Tea Extract on Human Osteosarcoma Cell Line MNNG-HOS Xenografts in Nude Mice: Evaluation of Tumor Growth and Immunohistochemistry

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2006, 23(3): 411-417

Introduction:
Structural changes in the extracellular matrix (ECM) are necessary for cell migration during tissue remodeling. MMPs, VEGF, Ki 67 (proliferative protein), and constituents of ECM play a critical role in angiogenesis and underlie neoplastic invasion and metastasis.

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Antitumor Effect of Nutrient Synergy on Human Osteosarcoma Cells U2OS, MNNG-HOS, and Ewing’s Sarcoma SK-ES.1

M.W. Roomi, V.Ivanov, T.Kalinovsky, A. Niedzwiecki, M.Rath
Oncology Reports 2005, 13(2): 253-257

Abstract:
Current treatment of osteosarcoma is associated with poor prognosis, especially due to the increased risk of developing other cancers with chemotherapy. Therefore, new safe effective treatment strategies are needed. We investigated the effect of a unique mixture of nutrients containing: lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate (EGCG) on human osteosarcoma cell lines: U-2OS, MNNG-HOS, and Ewing SK-ES-1 by measuring: cell proliferation, expression of MMP-2, MMP-9, and invasive and angiogenesis potential.

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