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Inhibition of tumor growth and metastasis by a novel nutrient mixture of inoculation of mouse mammary 4T1 carcinoma in kidney of female Balb/c mice

M.W. Roomi, Ph.D., B. Bhanap M.D., A. Niedzwiecki, Ph.D. and M. Rath, M.D.

Dr. Rath Research Institute, Santa Clara, California, USA
Journal of Cellular Medicine and Natural Health, June 2018

 

Abstract:

Breast cancer is the most prevalent and commonly diagnosed cancer in women worldwide. Though it is treatable in early stages, there is no effective treatment available once it metastasizes. Renal cell carcinoma (RCC) incidence is also on the rise and most of the patients present with metastatic cancer at the time of diagnosis. RCC is frequently presented with lung metastases that are poorly responsive to the conventional treatment of chemotherapy, and hormone and radiation therapy.

We studied the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, green tea extract, and quercetin on inhibition of tumor growth and metastasis by inoculation of mouse mammary carcinoma 4T1 cells in the kidney of female BALB/c mice. The 5- to 6-weeks-old BALB/c mice (n=10), were inoculated in the left kidney with 5 x 105 4T1 cells in 100 µl media. The right kidney was left untreated. After injection, the mice were randomly divided into two groups. The control group of mice (n = 5) were fed a regular mouse chow diet and the NM group (n = 5) was given the same diet but supplemented with 0.5% NM. After two weeks the mice were sacrificed and the abdominal cavities were opened. Both kidneys and other organs were excised and examined for metastasis. The left kidney in the control group was significantly enlarged due to 4T1 tumor growth. In contrast, the left kidney in the NM group was less enlarged. Severe lung and spleen metastases were observed in the control group and mild metastasis was seen in the lungs and spleen of the NM group. The right kidney in both the control and NM group was unaffected. Neither group showed any metastatic lesions in the liver or heart. In conclusion, these results suggest that the NM has the potential to suppress tumor growth and tumor metastasis to the lungs and spleen.

Key Words: lipoprotein(a), Lp(a)+;Gulo (-/-) mice, vitamin C, 4T1 breast cancer cells, tumor growth, metastasis

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