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A nutrient mixture suppresses carbon tetrachloride induced acute hepatic toxicity in ICR mice |
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M.W. Roomi, T. Kalinovsky, N.W. Roomi, V. Ivanov, M. Rath, A. Niedzwiecki
Human & Experimental Toxicology 2008; 27: 559-566
We examined the effect of a nutrient mixture (NM) that contains lysine, proline, ascorbic acid and green tea extract in mice treated with carbon tetrachloride (CCl4), a model of liver injury in which free radical, oxidative stress and cytokine production are closely linked. Seven-week-old male ICR mice were divided into four groups (A-D) of five animals each. Groups A and C mice were fed a regular diet for two weeks while groups B and D mice were supplemented with 0.5% NM (w/w) during that period. Groups A and B received corn oil ip, while groups C and D received CCl4 (25 L/kg, in corn oil, ip). All animals were sacrificed 24 h after CCl4 administration, serum was collected to assess liver and kidney functions, and livers and kidneys were excised for histology. Mean serum AST and ALT were comparable in groups A and B, increased markedly in group C and significantly lower in group D compared to group C. CCl4 had no significant effect on renal markers (BUN, creatinine and BUN/creatinine ratio). CCl4 administration caused an intense degree of liver necrosis that was less severe in the NM fed group D. These results indicate that NM could be a useful supplement in preventing acute chemical-induced liver toxicity.
Key Words:
Carbon tetrachloride, hepatic, toxicity, nutrients, ICR mice
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het_2008_27_p559.pdf