|
Inhibitory effects of a novel nutrient mixture on MMP secretion and invasion on human thyroid cancer cell line SW 579 |
 |
 |
 |
M.W. Roomi, B. Bhanap, A. Niedzwiecki, and M. Rath
JANA 2009, 13(1): 26-34
Abstract:
Thyroid cancer is the most common endocrine malignancy. Mortality from thyroid cancer results from tumor invasion with local and distant metastasis. Degradation of extracellular matrix is the hallmark of metastasis and is mediated by matrix metalloproteinase (MMP) enzymes. A novel nutrient mixture (NM) containing ascorbic acid, lysine, proline and green tea extract has exhibited significant anticancer activity in other cancer cell lines. In this study, we investigated the effect of NM on a thyroid cancer cell line on proliferation (MTT assay), MMP secretion (gelatinase zymography, Matrigel invasion, and morphology (H&E). Zymography demonstrated that NM inhibited MMP-2 secretion, with virtually total inhibition at 1000 μg/ml. Matrigel invasion was inhibited at 50, 100, and 500 μg/ml by 42%, 63% and 100% respectively. NM was nontoxic to thyroid cancer cells below 500 μg/ml and H&E staining did not show morphological changes.
Key Words:
Thyroid cancer, MMPs, Matrigel™ invasion, nutrients, green tea extract, ascorbic acid, lysine.
|
jana_2009_13_1_26.pdf