Modulation of u-PA, MMPs and their inhibitors by a novel nutrient mixture in human female cancer cell lines

M.W. Roomi, T. Kalinovsky, M. Rath and A. Niedzwiecki
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Oncology Reports 2012, 28:768-776

Abstract:
Cancers of the breast, cervix, uterus and ovary are the most prevalent cancers in women worldwide. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of urokinase plasminogen activators (u-PA) and matrix metalloproteinases (MMPs) with cancer progression, metastasis and shortened patient survival. MMP activities are regulated by specific tissue inhibitors of metalloproteinases (TIMPS).

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Anticancer Effects of a Specific Mixture of Nutrients in Multidrug Resistant Human Uterine Sarcoma Cell Line MES-SA/Dx5 and in Drug Sensitive MES-SA Cell Line

 

M.W. Roomi, T. Kalinovsky, N.W. Roomi, M. Rath and A. Niedzwiecki
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Oncology Reports 2011, 27: 17-27

Abstract:
A specific nutrient mixture containing lysine, proline, ascorbic acid and green tea extract (NM) has demonstrated a broad spectrum of antitumor activity against a number of cancer cell lines. In this study, our main objective was to investigate the comparative effects of NM on anticancer parameters such as cytotoxicity, MMP secretion, and Matrigel invasion in the human uterine sarcoma drug-resistant MES-SA/Dx5 and drug sensitive drug-sensitive MES-SA cell lines. In addition we studied the effect of NM on P-glycoprotein (Pgp) on these cell lines.

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In Vitro Modulation of MMP-2 and MMP-9 in Human Cervical and Ovarian Cancer Cell Lines by Cytokines, Inducers, and Inhibitors

M.W. Roomi, J.C. Monterrey, T. Kalinovsky, M. Rath, A. Niedzwiecki
Oncology Reports 2010, 23: 605-614

Abstract:
Matrix  metalloproteinases (MMPs) secreted by cervical and ovarian cancer, especially  MMP-2 and MMP-9, play crucial roles in tumor invasion and metastasis. We examined the effect of cytokines, mitogens, inducers and inhibitors on MMP-2 and MMP-9 expression in cervical and ovarian cancer cell lines. Human cervical (Hela and DoTc2-4510) and ovarian (SK-OV-3) cell lines were cultured in appropriate media. At near confluence, the cells were washed with PBS and incubated in serum-free medium with various concentrations of several cytokines, mitogens and inhibitors. After 24h the media were removed and analyzed for MMP-2 and MMP-9 by gelatinase zymography and quantitated by densitometry.

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Suppression of Human Cervical Cancer Cell Lines Hela and DoTc2 4510 MMP Expression and Matrigel Invasion by a mixture of lysine, proline, ascorbic acid, and green tea extract

M.W. Roomi, V. Ivanonv, T. Kalinovsky, A. Niedzwiecki, M. Rath
International Journal of Gynecological Cancer 2006, 16(3): 1241-1247

Introduction:
Cervical cancer, the second most common cancer in women, once metastasized, leads to poor prognosis. We investigated the antitumor effect of a mixture (NM) containing lysine, proline, arginine, ascorbic acid, and green tea extract on human cervical cancer cells Hela (CCL2) and DoTc2 4510.

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Inhibition of Matrix Metalloprotenase-2 Secretion and Invasion by Human Ovarian Cancer Cell Line SK-OV-3 with Lysine, Proline, Ascorbic Acid, and Green Tea Extract

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Journal of Obstetrics and Gynaecology Research 2006, 32(2):148-154.

Introduction:
Based on the poor prognosis associated with ovarian cancer and reported anti-cancer properties of specific nutrients, we investigated the effect of a nutrient mixture (NM) containing lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on human ovarian cancer cells SK-OV-3 by measuring: cell proliferation, modulation of MMP-2 and –9 secretion, and cancer cell invasive potential.

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