Chlorophyllin Suppresses Growth, MMP Secretion, Invasion and Cell Migration of Fibrosarcoma Cell LineHT1080

M.W. Roomi, B Bhanap, A Niedzwiecki, M Rath

Department of Oncology, Dr. Rath Research Institute, USA

Medical Research Archives, v. 6, n. 12, dec. 2018.

Abstract: 

Fibrosarcoma is an aggressive and highly malignant cancer of connective tissue with the lung being the most common site of metastasis. Surgery, chemotherapy, and radiation are the mainstay of treatments, yet the prognosis is very poor. A number of plant-based phytochemicals are increasingly being used as important treatment methods of cancers.

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A Nutrient Mixture Induces Caspase Dependent Apoptosis in Human Synovial Sarcoma Cells

M.W. Roomi, B Bhanap, Ahmed T, A Niedzwiecki, M Rath

Department of Oncology, Dr. Rath Research Institute, USA

J Sarcoma Res. 2018; 2(1): 1010 -1014

Abstract: 

Synovial sarcoma is a rare soft tissue cancer occurring more frequently in adolescent and young adults than older people. The primary sites being the joints of the arms and the legs.A novel Nutrient Mixture (NM) containing green tea extract, ascorbic acid, lysine, and proline exhibited anti-cancer effects in various cancers. In our earlier studies, the NM considerably reduced the tumor weight and tumor burden in synovial sarcoma.

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Anti-cancer potential of a specific mixture of phytonutrients in bone cancer cells

M.W. Roomi, B Bhanap, A Niedzwiecki, M Rath

Journal of Cellular Medicine and Natural Health Oct 2017

Abstract: 

Chondrosarcoma, a malignant tumor of cartilaginous origin, is the most common of the adult primary bone cancers. Forty percent of primary bone cancers are chondrosarcoma. However as chondrosarcoma typically does not respond to radiation and chemotherapy, surgery is the main treatment option. Cancer mortality usually results from the local and distant metastasis. Based on the poor prognosis of chondrosarcoma, we investigated effects of a phytonutrient mixture (PB) containing quercetin, CruciferexTM, curcumin, resveratrol and green tea extract on several parameters associated with the development of this type of cancer.

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Apoptosis of Human Fibrosarcoma Cells HT-1080 Triggered by a Novel Nutrient Mixture via Induction of Caspases

M.W. Roomi, B. Bhanap, A. Niedzwiecki, M. Rath

Sarcoma Res Int. 2016; 3(3): 1036

 

Abstract: 

Fibrosarcoma is an aggressive and highly malignant cancer of connective tissue with the lung being the most common site of metastasis. Surgery, chemotherapy, and radiation are the mainstay of treatments, yet the prognosis is very poor. A novel Nutrient Mixture (NM) containing green tea extract, ascorbic acid, lysine, and proline exhibited anti-cancer effects in various cancers.

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Modulation of u-PA, MMPs and their inhibitors by a novel nutrient mixture in adult human sarcoma cell lines

M.Waheed Roomi, Tatiana Kalinovsky, Aleksandra Niedzwiecki* and Matthias Rath
Dr. Rath Research Institute, Santa Clara, CA
International Journal of Oncology 2013; 43: 39-49

Abstract: 
Adult sarcomas are highly aggressive tumors that are characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the ECM and basement membrane, allowing cancer cells to spread to distal organs. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of u-PA and MMPs with cancer progression, metastasis and shortened patient survival. MMP activities are regulated by specific tissue inhibitors of metalloproteinases (TIMPs).

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Modulation of u-PA, MMPs and their inhibitors by a novel nutrient mixture in pediatric human sarcoma cell lines

M.Waheed Roomi, Tatiana Kalinovsky, Aleksandra Niedzwiecki* and Matthias Rath
Dr. Rath Research Institute, Santa Clara, CA
International Journal of Oncology 2013; 43: 1027-1035

Abstract:
Pediatric sarcomas are highly aggressive tumors that are characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the ECM and basement membrane, allowing cancer cells to spread to distal organs. Proteases play a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. Strong clinical and experimental evidence demonstrates association of elevated levels of u-PA and MMPs with cancer progression, metastasis and shortened patient survival. MMP activities are regulated by specific tissue inhibitors of metalloproteinases (TIMPs).

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In vitro modulation of MMP-2 and MMP-9 in adult human sarcoma cell lines by cytokines, inducers, and inhibitors

M.W. Roomi, T. Kalinovsky, J. Monterrey, M. Rath, A. Niedzwiecki
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
International Journal of Oncology 2013, DOI: 10.3892/ijo.2013.2113

Abstract
The highly aggressive adult sarcomas are characterized by high levels of matrix metalloproteinase (MMP)-2 and -9, which play crucial roles in tumor invasion and metastasis by degradation of the extracellular membrane leading to cancer cell spread to distal organs. We examined the effect of cytokines, mitogens, inducers and inhibitors on MMP-2 and MMP-9 secretion in chondrosarcoma (SW-1353), fibrosarcoma (HT-1080), liposarcoma (SW-872) and synovial sarcoma (SW-982) cell lines.

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The Anti-Cancer Effect of a Novel Nutrient Mixture by Inhibiting MMPs Expression, Invasion and Inducing Apoptosis in Chondrosarcoma Cell Line SW-1353

M W Roomi, N W Roomi, B Bhanap, A Niedzwiecki, M Rath;                                            

Cancer and Clinical Oncology 2012; Vol. 1, No. 2,

 

Abstract: 

primary bone cancers. Surgery is the main treatment option because chondrosarcoma typically does not respond to radiation and chemotherapy. Cancer mortality usually results from the local and distant metastasis. A nutrient mixture containing ascorbic acid, lysine, proline, green tea extract, was tested on chondrosarcoma cells SW-1353 for viability, matrix metalloproteinase expression, Matrigel invasion, morphology and apoptosis.

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In vitro anticarcinogenic effect of a nutrient mixture on human rhabdomyosarcoma cells

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Gene Therapy and Molecular Biology 2007, 11: 133-142

Abstract:
Rhabdomyosarcoma, the most common pediatric soft tissue sarcoma of mesenchymal origin, has metastasized in ~25% of all patients at time of diagnosis. Though current treatment strategies have achieved some success, they are associated with severe adverse effects. We investigated the effect of a nutrient mixture (NM), which has shown antitumor effects on various cancer cell lines, on rhabdomyosarcoma cell growth, apoptosis, MMP secretion, and invasion. Human rhabdomyosarcoma cells, grown in DME, were treated at near confluence with NM at 0, 10, 50, 100, 500 and 1000 μg/ml in triplicate at each dose.

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Inhibition of Cell Invasion and MMP Production by a Nutrient Mixture in Malignant Liposarcoma Cell Line SW-872

M.W. Room, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2007, 24: 394-401

Abstract:
Liposarcoma, a malignancy of fat cells, is the most common soft tissue sarcoma. Though rare, poorly differentiated liposarcomas commonly metastasize to lungs and liver, leading to poor prognosis. Prevention of Extracellular matrix (ECM) degradation by inhibition of matrix metalloproteinases (MMPs) activity has been shown to be a promising therapeutic approach to inhibition of cancer progression. A nutrient mixture (NM) containing lysine, proline, ascorbic acid, and green tea extract has shown significant anticancer activity against a number of cancer cell lines. We investigated the effect of NM on liposarcoma cell line SW-872 proliferation (MTT assay), MMP secretion (gelatinase zymography), invasion through Matrigel, and apoptosis and morphology (live green caspase kit and H&E).

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In Vivo and In Vitro Antitumor Effect of Ascorbic Acid, Lysine, Proline, Arginine and Green Tea Extract on Human Fibrosarcoma Cells HT-1080

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Medical Oncology 2006, 23(1): 105-112

Introduction:
Current treatment of fibrosarcoma, an aggressive cancer of the connective tissue, is generally associated with poor prognosis. Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and constituents of the ECM, such as fibronectin, play a critical role in angiogenesis and underlie neoplastic invasion and metastasis. This and anticancer properties of lysine, proline, arginine, ascorbic acid, and green tea extract (NM) prompted us to investigate the effect of these nutrients in vitro on human fibrosarcoma cells HT-1080 by measuring: cell proliferation, modulation of MMP-2 and MMP-9, and invasive potential.

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In Vitro and In Vivo Antitumor Effect of a Nutrient Mixture Containing Ascorbic Acid, Lysine, Proline, and Green Tea Extract on Human Synovial Sarcoma Cancer Cells

 

M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
Journal of the American Nutraceutical Association 2006, 9(2): 30-34

Introduction:
Synovial sarcoma, a soft tissue cancer that most often occurs around leg or arm joints, has a 50% rate of metastasis. Structural changes in the extracellular matrix (ECM) are necessary for cancer cell migration and invasion.

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Synergistic Antitumor Effect of Ascorbic Acid, Lysine, Proline, and Epigallocatechin Gallate on Human Fibrosarcoma Cells HT-1080

M.W. Roomi, V.Ivanov, T.Kalinovsky, A. Niedzwiecki, M.Rath
Annals of Cancer Research and Therapy 2004, 12(1-2): 146-156

Abstract:
Matrix metalloproteinases (MMPs) have received much attention in recent years for their role in various malignancies, and have been implicated in tumor invasion, metastasis and angiogenesis. Certain MMPs, such as MMP-2 and MMP-9, have shown a special propensity for promoting cancer. Development of MMP inhibitors has been a recent approach to controlling cancer and blocking metastasis.

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