Progress of Tumor Growth and Metastasis After Inoculation of B16FO Melanoma Cells in Kidney of Female Nude Mice Is Inhibited by a Novel Nutrient Mixture

M. Waheed Roomi, Bilwa Bhanap, Aleksandra Niedzwiecki, Matthias Rath
Dr. Rath Research Institute, Santa Clara, California, USA
Integrative Cancer Therapies Volume 18: 1–8 DOI: 10.1177/1534735419832365

Abstract:
Tumor metastasis is a major cause for most cancer-related deaths. Melanoma is serious cancer that metastasizes to other areas of the body, including the lungs, liver, brain, bones, or lymph nodes. Currently used cancer therapies are ineffective with a high degree of toxicity and patient mortality. Thus, any successful treatment for melanoma must target metastasis.

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Modulation of MMP-2 and -9 Secretion by Cytokines, Inducers and Inhibitors in Human Melanoma A-2058 Cells

Waheed Roomi, Tatiana Kalinovsky, Aleksandra Niedzwiecki, Matthias Rath
Dr. Rath Research Institute, Santa Clara, California, USA
Oncology ReportsDOI: 10.3892/or.2017.5597

Abstract:
Melanoma, an extremely aggressive cancer, causes the most skin cancer-related deaths, due to metastasis to other areas of the body, such as lymph nodes, lungs, liver, brain or bone. It is characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the extracellular matrix and basement membrane, allowing cancer cells to spread to distal organs.

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Suppression of metastasis of intratesticular inoculation of B16FO melanoma cells by a novel nutrient mixture in male athymic nude mice

M.W. Roomi, T. Kalinovsky, N.W. Roomi,  A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental and Therapeutic Medicine 2012; 4: 775-780

Abstract:
Metastasis, commonly to the lung, is the major cause of death from testicular cancer. We studied the effect of a novel nutrient mixture (NM) containing ascorbic acid, amino acids and green tea extract on inhibition of melanoma growth and metastasis using a model of intratesticular inoculation of B16FO cells into nude mice. Male athymic mice (n=12), 10-12 weeks of age, were inoculated with 5X105 B16FO melanoma cells in 100 µL of PBS into the right testis; the left testis was left untreated. After inoculation, the mice were randomly divided into two groups. The Control group (n=6) was fed a regular mouse chow diet and the NM 1% group (n=6) the same diet but supplemented with 1% NM. Four weeks later the mice were sacrificed and the abdominal cavity was opened.

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Anticancer Effects of a Micronutrient Mixture on Melanoma: Modulation of Metastasis and Other Critical Parameters

Roomi MW, Kalinovsky T, Niedzwiecki A, Rath M
Breakthroughs in Melanoma Research, ed. Y. Tanaka, In Tech Publisher, 2011, Ch 26,  pp 559-574

Abstract:
Consumption of a plant-based diet has been associated with prevention of the development and progression of cancer. We have developed strategies to inhibit cancer development and its spread by targeting common mechanisms used by all types of cancer cells that decrease stability and integrity of connective tissue. Our approach to strengthening of collagen and connective tissue compromised in cancer metastasis , tumor growth and angiogenesis has been based on using specific natural components, among them  lysine, proline, ascorbic acid and green tea extract (NM). We have documented synergistic effects of this micronutrient mixture and its effectiveness in controlling key mechanisms of cancer  in vivo and in vitro in a wide variety of cancer cell lines.

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Ascorbate Depletion Increases Growth and Metastasis of Melanoma Cells in Vitamin C Deficient Mice

J. Cha, M.W. Roomi, V. Ivanov, T.Kalinovsky, A.Niedzwiecki, M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050
Experimental Oncology 2011, 33(4):1-5

Abstract:
Melanoma, an aggressive skin cancer, causes the most skin cancer-related deaths, due to metastasis to other areas of the body, Degradation of the extracellular matrix plays a critical role in the formation of tumors and metastasis and has been found to correlate with the aggressiveness of tumor growth and invasiveness of the cancer. Vitamin C, synthesized by many animals but not by humans, is known to be essential for the structural integrity of the intercellular matrix. Humans must obtain ascorbate by diet and cancer patients have been shown to have very low reserves of vitamin C.

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Inhibition of 7,12-dimethylbenzanthracene-induced skin tumors by a nutrient mixture

M.W. Roomi, N.W. Roomi, T. Kalinovsky, V. Ivanov, M. Rath, A. Niedzwiecki
Medical Oncology 2008, 25(3): 333-340

Abstract:
The annual incidence of all forms of skin cancer, the most common of all human cancers, is increasing yearly. A unique nutrient mixture (NM) was shown to exhibit anticancer activity in vivo and in vitro. We examined the effect of NM on the development of skin cancer induced by 7,12-dimethylbezanthracene (DMBA) in female SENCAR mice by a complete carcinogenesis protocol. Mice (n = 55) were divided into four groups and carefully shaved on dorsum.

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In Vitro and In Vivo Antitumor Effect of Ascorbic Acid, Lysine, Proline, And Green Tea Extract On Human Melanoma Cell Line A2058

M.W. Roomi, N. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath
In Vivo 2006; 20(1):25-32

Introduction:
Melanoma, a very serious form of skin cancer, causes the most skin cancer-related deaths, due to metastasis. Structural changes in the ECM are necessary for cell migration during tissue remodeling. MMPs, VEGF, Ki 67 (proliferative protein), and constituents of ECM play a critical role in angiogenesis, and are crucial in neoplastic invasion and metastasis. Based on antitumor properties of certain nutrients, we investigated the effect of a diet containing lysine, proline, arginine, ascorbic acid, and green tea extract on the growth of tumors induced by implanting human melanoma A2058 cells in athymic nude mice and on the expression of MMPs, VEGF, Ki 67 and fibronectin in these tumors, as well as the production of mucin (by PAS staining).

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