| Essential
Nutrients Suppress Inflammation By Modulating Key Inflammatory
Gene Expression
V. Ivanov, J. Cha, S. Ivanova, M.W.Roomi, A. Niedzwiecki, M.Rath.
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: 8th World Congress in Inflammation;
Copenhagen, Denmark; June 16-20, 2007
Published in: Inflammation Research, volume
56, supplement 3, June 2007, page S471
Poster #P12.05
Abstract
Introduction:
The process of inflammation accompanies the progression and aggravates
the outcome of all modern human chronic pathological conditions.
There is a growing body of evidence of the beneficial role of
proper nutrition in controlling inflammation.
Objective:
We investigated whether supplementation with selected essential
nutrients would be beneficial in experimental inflammation and
the molecular mechanisms involved.
Materials and Methods:
The tested nutrient mixture (NM) consisted of green tea catechins,
citrus flavonoids hesperidin, naringenin and quercetin, ascorbate,
lysine, proline, arginine and cysteine. The development of systemic
inflammation in mice in response to challenge with bacterial lipopolysaccharide
(LPS) was monitored by blood plasma levels of fourteen key inflammatory
cytokines.
Results:
Two-week supplementation with 250 mg NM/kg body weight prior to
LPS challenge provided protection significantly greater than the
reference supplementation with ibuprofen. Taken as illustrative
example, the induction of the two cytokines most responsive to
LPS challenge, interleukin-6 (IL-6) and monocyte chemoattracting
protein-1, was reduced in NM-supplemented animals by 58% and 86%,
respectively, whereas corresponding reduction in ibuprofen group
was 34% and 43%. Protective mechanisms involved were assessed
in human cultured U937 macrophages stimulated with LPS. The most
responsive cytokines from the panel in this model were tumor necrosis
factor alpha (71% and 17% reduction by supplementation with NM
and ibuprofen, respectively) and IL-12 (66% and 15% in corresponding
reduction). NM supplementation dramatically reduced prostaglandin
E2 secretion by stimulated macrophages along with cyclooxigenase-2
(COX2) cellular protein expression. mRNA levels for COX2 and inflammatory
cytokines were also dramatically reduced. Quercetin was the most
effective nutrient when tested individually. However, NM appeared
to surpass the combined effects of individual components.
Conclusion:
We conclude that the combination of essential nutrients demonstrates
strong beneficial effects in experimental inflammation model by
targeting responsible gene expression.
Comment:
Inflammation accompanies the progression and aggravates the
outcome of all modern human chronic pathological conditions.
Much evidence suggests that proper nutrition helps control
inflammation. We investigated whether supplementation with
a mixture of selected essential nutrients (NM) consisting
of green tea catechins, citrus flavanoids hesperidin, naringenin
and quercetin, ascorbate, lysine, proline, arginine and cysteine
would be beneficial in bacterial liposaccharide (LPS)-induced
systemic inflammation in mice. We monitored blood plasma levels
of fourteen key inflammatory cytokines. Supplementation with
NM prior to LPS challenge provided significantly greater protection
than supplementation with ibuprofen. Supplementation with
NM dramatically reduced prostaglandin E2 secretion by stimulated
macrophages along with cyclooxigenase-2 (COX2) cellular protein
expression. mRNA levels for COX2 and inflammatory cytokines
were also dramatically reduced. These results are significant
since they illustrate the superior protective anti-inflammatory
effect of supplementation with the non-toxic nutrient mixture
over the commonly used drug ibuprofen with its adverse effects. |
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