| Nutrient
Mixture Prevents Hyper-Reactive Response Of Bronchial Muscle To
Inflammatory And Allergic Stimuli.
Ivanov V, Roomi MW, Niedzwiecki A, Rath M
Matthias Rath Research Institute BV, Santa Clara, CA.
Presented at: 46th Annual Meeting of the American
College of Nutrition, Charleston, South Carolina, Sept 22-25,
2005.
Published in: Journal of the American College
of Nutrition, vol 24(5), Oct 2005, abstract #74
Abstract
Introduction:
Asthma, a chronic pathological condition poorly managed by conventional
treatment, is manifested by bronchial hyper-reactivity and bronchial
spasm. Pathogenesis is linked to infection, inflammation, and
allergic reaction to pollution.
Objective:
We investigated the effect of a nutrient mixture (NM) consisting
of ascorbic acid, amino acids lysine, proline, arginine and cysteine,
and polyphenols from green tea extract on bronchial muscle hyper-reactive
response to pathogenic stimuli in vitro.
Methods:
We investigated the effect of a nutrient mixture (NM) on bronchial
muscle hyper-reactive response to pathogenic stimuli in vitro,
using the model of cultured human bronchial smooth muscle cells
(BrSMC) embedded in three-dimension collagen type I gel.
Results:
Gel contraction was accelerated by K+, Ca2+ and Mg2+ ions and
decreased by bivalent metal chelators, such as EDTA and heparin.
Stimulation of BrSMC with inflammatory mediators as bacterial
lipopolysaccharide (LPS) and endothelin-1 significantly increased
collagen gel contraction. Histamine, the principal mediator of
allergic reaction, accelerated BrSMC-dependent collagen gel contraction
in a dose-dependent manner. NM inhibited the hyper-reactive response
of BrSMC to all tested pathogenic stimuli in a dose-dependent
manner. The relaxation effect of NM was greater than the combined
effect of its individual components.
Conclusion:
In conclusion, NM has therapeutic potential in safely and effectively
controlling asthma. These data justify further in vivo experimental
and clinical testing.
Comments
Asthma, a chronic pathological condition manifested by bronchial
hyper-reactivity and bronchial spasm is poorly managed by
conventional treatment. Pathogenesis is linked to infection,
inflammation, and allergic reaction to pollution. We found
that a nutrient mixture of ascorbic acid, amino acids lysine,
proline, arginine and cysteine, and polyphenols from green
tea extract significantly inhibited the hyper-reactive response
of bronchial smooth muscle cells in vitro to histamine and
to inflammatory mediators, such as bacterial lipopolysaccharides
and endothelin-1. Furthermore, the relaxation effect of the
nutrient mixture was greater than the combined effect of its
individual components. These findings are significant as they
indicate that nutrient synergy has therapeutic potential in
safely and effectively controlling asthma. |
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