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Novel Nutrient Mixture Induces Apoptosis in Human Prostate Cancer
Cell Line Du-145
M.W. Roomi, N.W. Roomi, V. Ivanov, A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: Apoptosis in Drug Discovery; San
Diego, CA; March 22-23, 2007
Published in: Proccedings of the GTCbio Conference
on Apoptosis in Drug Discovery
Abstract
Introduction:
Prostate cancer, the major cancer affecting males and second most
deadly cancer in the United States, primarily affects males aged
55 and older and African Americans more than Caucasians. Once
prostate cancer has metastasized, current treatment methods are
generally ineffective. We have identified a novel antineoplastic
agent, a nutrient mixture (NM) containing lysine, proline, ascorbic
acid and green tea extract that demonstrates a broad spectrum
of antitumor activity against a number of human cancer cell lines.
Objective:
We investigated whether the cytotoxic effect of NM on DU145 was
consistent with its apoptotic effect on these cells.
Method and Materials:
Prostate cancer DU-145 (ATCC) cells were cultured in MEM and treated
with NM in different concentrations, 0, 100, 500 and 1000 µg/ml
concentration. Growth inhibition was detected by MTT assay, morphology
by H&E staining, and apoptosis by Live Green Caspases Detection
Kit.
Results:
NM was slightly toxic to DU145 cells at 100-µg/ml and profound
at 500 and 1000 µg/ml. DU-145 cells treated with NM at 500
and 1000 µg/ml concentration and stained by H&E demonstrated
obvious apoptotic cells. Apoptotic cells showed shrinkage with
darkly stained and condensed nuclei and strongly acidophilic cytoplasm.
Using caspases kit, slight apoptosis was observed at 100 µg,
moderate at 500 and significant at 1000 µg/ml concentrations.
Conclusions:
The results suggest that NM induces apoptosis in prostate cancer
cell line DU-145 and raises the possibility of its use as a new
chemotherapeutic a gent.
Comment:
A novel antineoplastic agent, a nutrient mixture (NM) containing
lysine, proline, ascorbic acid and green tea extract demonstrates
a broad spectrum of antitumor activity against a number of
human cancer cell lines. We investigated whether the cytotoxic
effect of NM on DU145 was consistent with its apoptotic effect
on these cells. DU-145 cells treated with NM at 500 and 1000
µg/ml demonstrated profound toxicity and obvious apoptotic
cells morphologically. Using caspases kit, slight apoptosis
was observed at 100 µg, moderate at 500 and significant
at 1000 µg/ml concentrations. The results are significant
as they suggest NM as a therapeutic agent for prostate cancer,
the major cancer affecting males and second most deadly cancer
in the United States. |
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