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Antineoplastic
Effect of Nutrient Mixture on Human Burkitt’s Lymphoma Raji
Cells
M.W. Roomi, N.W. Roomi, V. Ivanov, A. Niedzwiecki
and M. Rath
Dr. Rath Research Institute, Oncology Division, Santa Clara, CA
95050
Presented at: 97th Annual Meeting of the AACR,
Washington D.C., April 1-5, 2006
Published in: Proceedings of the 97th Annual
Meeting of the AACR, Abstract #1915
Abstract
Introduction:
Current treatment for Burkitt’s lymphoma (BL) utilizes combined
cytotoxic chemotherapy. However, this treatment is highly toxic
and is markedly less successful in AIDS-related BL. Therefore,
additional therapy is urgently required. We previously demonstrated
that a unique non-toxic nutrient mixture (NM) consisting of lysine,
proline, ascorbic acid and green tea extract has antineoplastic
activity against a number of cancer cell lines both in vitro and
in vivo.
Objective:
In the present study we investigated the antineoplastic activity
of NM on human Burkitt’s lymphoma Raji cells, evaluating
cell proliferation, MMP secretion, and invasion through Matrigel.
Method and Materials:
Human Burkitt’s lymphoma Raji cells (ATCC) were grown in
RPMI-1640 medium with 10% fetal bovine serum and antibiotics
and
treated with NM at 0, 10, 50, 100, 500 and 1000 µg/ml concentration
in triplicate at each dose. Cell proliferation was assessed by
counting cells stained with Trypan blue, invasion was evaluated
through Matrigel, and MMP activity by gelatinase zymography. Cells
were also treated to induce MMP-9 activity.
Results:
NM exhibited a dose response toxicity of 30%, 40%, 60%, and 90%
at 10, 50, 100, and 500 µg/ml concentration respectively. Zymography
demonstrated production of only MMP-9, which was further induced
by PMA treatment. NM showed a significant decrease in MMP-9 expression
at 500 µg/ml, which was totally abolished at 1000 µg/ml concentration.
Invasion through Matrigel was inhibited at 10, 50 and 100 µg/ml
by 60%, 85% and 100% respectively.
Conclusions:
Our results suggest that NM is a promising agent to be considered
in the current treatment of BL in endemic and AIDS related diseases.
Comment:
Chemotherapy, the current treatment for Burkitt’s lymphoma
(BL) is toxic and has limited success in AIDS-related forms.
We investigated the antineoplastic effect of a nutrient mixture
(NM) on BL in vitro. NM significantly inhibited BL cellular
proliferation, MMP-9 secretion, and invasion through Matrigel,
with total block of invasion at 100 µg/ml NM. These results
are significant as they indicated NM has strong potential
in treatment of BL. |
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