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Inhibitory Effect of a Novel Nutrient Mixture
on MMP Secretion and Invasion on Human Thyroid Cancer Cell Line
SW 579: In Vitro Study
M.W. Roomi, N.W. Roomi, V. Ivanov, A. Niedzwiecki
and M. Rath
Dr. Rath Research Institute, Oncology Division, Santa Clara, CA
95050
Presented at: AICR International Research Conference
on Food, Nutrition and Cancer, July 13-14, 2006, Washington DC
Published in: AICR International Research Conference on Food, Nutrition and Cancer Poster Abstract Book, Abstract #42
Abstract
Introduction:
Thyroid cancer, the most common type of endocrine malignancy,
accounts for more than 90% of all endocrine tumors. In 2004, 24,000
new thyroid cancers were diagnosed and 1460 deaths resulted, due
to complications of the disease or treatment. Cancer mortality
results mainly from the tumor invading local tissues and metastasizing
to vital organs such as the liver, lung, brain etc. Degradation
of the ECM, a hallmark of these events, is mediated by MMPs. A
novel nutrient mixture (NM) containing ascorbic acid, lysine,
proline and green tea extract has exhibited MMP inhibition and
significant anticancer activity against a number of cancer cell
lines.
Objective:
We investigated the effect of NM on human thyroid cancer cell
SW 579 for viability, MMP secretion, invasion and morphology.
Methods:
Human thyroid cancer cell line SW 579 (ATCC) was grown in Liebowitz
medium with 10% FBS, penicillin and streptomycin in 24-well tissue
culture plates. At near confluence, cells were treated with NM
dissolved in media at 0, 10, 50, 100, 500 and 1000 µg/ml
in triplicate at each dose. Cell proliferation was assayed by
MTT assay, MMP secretion by zymography, invasion through Matrigel
and morphology by H&E staining.
Results:
Zymography demonstrated secretion of MMP-9, which showed a marked
decrease at 500 µg/ml and at 1000 µg/ml NM. Invasion
through Matrigel was inhibited at 50, 100 and 500 µg/ml
by 40%, 62% and 100% respectively. Interestingly NM was not toxic
to the cells. H&E staining showed no morphological changes
below 500 µg /ml.
Conclusions:
In conclusion, NM has a great potential for therapeutic use in
the treatment of thyroid cancer, by inhibiting critical steps
in cancer development such as MMP secretion and invasion.
Comment:
Mortality from thyroid cancer, the most common type of endocrine
malignancy, results mainly from the tumor invading local tissues
and metastasizing to vital organs such as the liver, lung,
and brain. Metastasis depends upon degradation of the ECM
and is mediated by MMPs. A novel nutrient mixture (NM) containing
ascorbic acid, lysine, proline and green tea extract has exhibited
MMP inhibition and significant anticancer activity against
a number of cancer cell lines. Our in vitro study on the effect
of NM on thyroid cancer cells SW 579 demonstrated inhibition
of invasive parameters such as MMP secretion and Matrigel
invasion, without toxicity to cells, indicating NM has therapeutic
potential in treatment of thyroid cancer. |
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