 |
|
|
Scientific Presentations - Other Research
 |
Essential
Nutrients Suppress Inflammation By Modulating Key Inflammatory
Gene Expression
- NEW -
V. Ivanov, J. Cha, S. Ivanova, M.W.Roomi,
A. Niedzwiecki, M.Rath.
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: 8th World Congress in Inflammation;
Copenhagen, Denmark; June 16-20, 2007
Published in: Inflammation Research, volume
56, supplement 3, June 2007, page S471
Poster #P12.05 |
| |
Inflammation
accompanies the progression and aggravates the outcome of
all modern human chronic pathological conditions. Much evidence
suggests that proper nutrition helps control inflammation.
We investigated whether supplementation with a mixture of
selected essential nutrients (NM) consisting of green tea
catechins, citrus flavanoids hesperidin, naringenin and quercetin,
ascorbate, lysine, proline, arginine and cysteine would be
beneficial in bacterial liposaccharide (LPS)-induced systemic
inflammation in mice. We monitored blood plasma levels of
fourteen key inflammatory cytokines. Supplementation with
NM prior to LPS challenge provided significantly greater protection
than supplementation with ibuprofen. Supplementation with
NM dramatically reduced prostaglandin E2 secretion by stimulated
macrophages along with cyclooxigenase-2 (COX2) cellular protein
expression. mRNA levels for COX2 and inflammatory cytokines
were also dramatically reduced. These results are significant
since they illustrate the superior protective anti-inflammatory
effect of supplementation with the non-toxic nutrient mixture
over the commonly used drug ibuprofen with its adverse effects. |
|
|
Isoniazid
Induces Apoptosis and Inhibits MMP Secretion in Human Hepatoma
Cell Line SK-Hep- 1
- NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: 46th Annual Meeting of Society
of Toxicology, Charlotte, NC, March 25-29, 2007
Published in: The Toxicologists (suppl
Toxicological Sciences), Abstract #1001, pg 207, 2007. |
| |
Isoniazid (INH),
a drug commonly used for treatment of tuberculosis, may lead
to liver injury in some patients. We studied the mechanisms
of INH toxicity using human hepatoma cell line SK-Hep-1. The
present study demonstrated that INH causes toxicity to SK-Hep-1
cells by inducing apoptosis and inhibiting MMP secretion. |
|
|
A
Nutrient Mixture Suppresses Carbon Tetrachloride Induced
Hepatic Toxicity in ICR Mice
- NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: FASEB, San Francisco, CA,
April 1-5, 2006
Published in: The FASEB Journal, Abstract
85 |
| |
Liver disease
and toxicity is common, especially with many drug treatments.
Carbon tetrachloride (CT)-induced liver injury in mice is
an excellent model to evaluate the effectiveness of agents
on inhibition or reversal of liver damage. A unique nutrient
formulation consisting primarily of lysine, proline, ascorbic
acid and green tea extract (NM) has demonstrated a broad spectrum
of pharmacological, therapeutic, cardiovascular and chemoprotective
properties. We examined the effect of a NM supplemented diet
on liver and kidney tissue and serum markers in ICR mice dosed
with CT. Administration of CT to mice on a regular diet resulted
in marked increase in serum AST and ALT levels (markers for
liver). In contrast, serum ALT and AST levels in NM-supplemented
mice were of low magnitude and comparable to control mice
(not dosed with CT). CT administration caused an intense degree
of liver necrosis that was less severe in the NM fed group.
These results are significant as they indicate that NM has
potential in treating and preventing liver disease. |
|
|
A
Nutrient Mixture Attenuates Acetaminophen Hepatic and Renal
Toxicity in ICR - NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: Society of Toxicology,
San Diego, CA, March 5-9, 2006
Published in: The Toxicologists (suppl
Toxicological Sciences) 90 (1), Abstract 1398, pg 285, 2006. |
| |
Overdosing
with acetaminophen, a common over-the-counter analgesic, is
often fatal, causing severe liver and kidney damage in humans
and animals. We tested the protective effect of a nutrient
supplemented diet (0.5% NM) in mice injected ip with acetaminophen
(600 mg/kg) using a nutrient mixture that has previously demonstrated
therapeutic and chemoprotective properties. The nutrient supplemented
mice were protected against acetaminophen-induced liver and
kidney damage, while the unsupplemented mice exhibited liver
and kidney pathology and associated serum enzyme abnormality.
Thus, the nutrient mixture provides protection against acetaminophen-induced
hepatic and renal damage. |
|
|
Nutrient
Mixture Prevents Hyper-Reactive Response of Bronchial Muscle
to Inflammatory and Allergic Stimuli. -
NEW -
Ivanov V, Roomi MW, Niedzwiecki A, Rath
M
Matthias Rath Research Institute BV, Santa Clara, CA.
Presented at: 46th Annual Meeting of
the American College of Nutrition, Charleston, South Carolina,
Sept 22-25, 2005.
Published in: Journal of the American
College of Nutrition, vol 24(5), Oct 2005, abstract #74 |
| |
Asthma, a chronic
pathological condition manifested by bronchial hyper-reactivity
and bronchial spasm is poorly managed by conventional treatment.
Pathogenesis is linked to infection, inflammation, and allergic
reaction to pollution. We found that a nutrient mixture of
ascorbic acid, amino acids lysine, proline, arginine and cysteine,
and polyphenols from green tea extract significantly inhibited
the hyper-reactive response of bronchial smooth muscle cells
in vitro to histamine and to inflammatory mediators, such
as bacterial lipopolysaccharides and endothelin-1. Furthermore,
the relaxation effect of the nutrient mixture was greater
than the combined effect of its individual components. These
findings are significant as they indicate that nutrient synergy
has therapeutic potential in safely and effectively controlling
asthma. |
|
|
Down
Regulation of MMP-2 Expression and Invasion Potential of Human
Gingival Fibroblasts (2004)
M.W.
Roomi, V. Ivanov, A. Niedzwiecki, M. Rath
Matthias Rath Research., Cancer Research Division, Santa Clara,
CA 95050
Presented at: XXVIII Scientific
Meeting of the South African Division of the International
Association for Dental Research, Pretoria, South Africa, September
2-3, 2004
Published in: Conference proceedings,
Abstract #37, pg 42 |
| |
Gingivitis,
the earliest stage of gum disease, affects three out of four
adults over the age of 35. Left untreated, it progresses to
periodontitis, an advanced stage of gum disease characterized
by additional destruction of gum tissue and progression to
the bone. MMPs play a critical role in this process as, in
concert, they degrade the majority of the extracellular matrix
components. We studied the effect of a combination (NM) of
ascorbic acid, lysine, proline, and green tea extract on expression
of MMPs by gingival fibroblasts in cell culture. NM significantly
inhibited MMP-2 expression and invasion through Matrigel,
suggesting NM has great potential as a therapeutic tool in
prevention and treatment of gingivitis and its sequelae. |
|
|
Natural
Nutrient Mixture of Ascorbic Acid with Amino Acids Lysine,
Arginine, Cysteine and Proline, and Green Tea Polyphenols
Effectively Reduces Collagen Matrix Contraction Driven by
Human Uterine Smooth Muscle Cells (2004)
V.Ivanov,
S. Ivanova, M. W. Roomi, A. Niedzwiecki, M. Rath
Matthias Rath BV, Research Institute, Santa Clara, CA, USA
Presented at: 12th International
Congress of Endocrinology, Lisbon, Portugal, August 31-September
4, 2004.
Published in: Conference proceedings,
Abstract #1166, pg 360. |
| |
Disturbances
in hormone regulation and inflammation-related processes have
been shown to play roles in abnormal uterine contractility,
which causes preterm delivery and can lead to perinatal morbidity
and mortality. We used an in vitro model of collagen I gel
contraction driven by embedded cultured human uterine smooth
muscle cells to study the effect of various nutrients on uterine
contraction. Among the bioflavonoids and plant extracts tested,
we found that green tea leaf extract and epigallocatechin
gallate showed the strongest ability to counteract uterine
smooth muscle cell contraction. Furthermore, addition of ascorbic
acid and amino acids lysine, arginine, cysteine and proline
to green tea extract further increased its effectiveness.
A reduction in gel contraction correlated with decreased MMP.
The results of this study are significant since they demonstrate
the great potential of nutrients in preventing preterm delivery
and its sequelae. |
|
|
Antiproliferative
Effects of Antioxidants Using HTLV-1 Positive and Negative
Malignant T-Cells (2004)
Harakeh SM,
Diab-Assaf M, Niedzwiecki A, Khalife J, Abu-El-Ardat K, Roomi
MW, Rath M.
Presented at: 104th General Meeting of
the American Society for Microbiology, New Orleans, Louisiana,
May 23-27, 2004.
Published in: American Society for Microbiology
Final Program, p 143, Abstract #T-021. |
| |
Adult T-cell
leukemia (ATL) is an aggressive malignancy caused by HTLV-1,
for which currently there is no proven therapy. Nutrients,
such as ascorbic acid, lysine, and EGCG used individually
and in combination with other nutrients (e.g., proline and
N-Acetyl-L-Cysteine), were effective in inhibiting the growth
of virus-infected and virus-negative leukemia cell types.
These nutrients were used in concentrations that did not affect
the viability of normal cells, but were effective in killing
leukemia cells. Our study results confirm our previous findings
that a specific combination of nutrients can be a powerful
tool in suppressing cancer cell growth and viability, including
blood cancers such as ATL. |
|
|
|
|
