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Scientific Presentations in Heart Disease
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Plant-Derived
Nutrients Inhibit Monocyte Retention By Extracellular Matrix
Produced By Aortic Smooth Muscle and Endothelial Cells
- NEW -
Vadim Ivanov, Svetlana Ivanova, M. Waheed
Roomi, Aleksandra Niedzwiecki, Matthias Rath.
Dr Rath Research Institute, Santa Clara, California, USA
Presented at: 76th Congress of the European
Atherosclerosis Society; Helsinki, Finland; June 10-13,
2007
Published in: Atherosclerosis Supplements,
volume 8, issue 1, June 2007, page 163, poster #P020-598 |
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Compromised
extracellular matrix (ECM) function and structure at lesion
sites contribute to monocyte retention within the vascular
wall and lead to the initiation and progression of atherosclerosis.
We investigated whether ECM biological properties would be
beneficially affected by exposure to plant-derived nutrients.
Of the nutrients tested, ascorbic acid, quercetin, epigallocatechin
gallate and asiatic acid demonstrated the most pronounced
effects individually, which was enhanced when nutrients were
combined. Monocyte attachment to ECM produced under supplementation
with the nutrient mixture was reduced in a dose-dependent
manner, with inhibition of moncyte attachment greater with
AoEC than AoSMC. The different patterns of AoEC- and AoSMC-derived
ECM attachment to monocytes were correlated with nutrient-induced
changes in the ECM composition. These results are significant
as they indicate that plant-derived nutrients affect ECM composition
and inhibit monocyte retention, and thus would be beneficial
in preventing progression of atherosclerosis. |
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Mixture
of Natural Nutients Reduces Collagen Matrix Contraction
Driven by Aortic Smooth Muscle Cells -
NEW -
V. Ivanov, S. Ivanova, M.W. Roomi, A.
Niedzwiecki, M. Rath
Matthias Rath Research Institute, 1260 Memorex Drive, Santa
Clara, CA 95050
Presented at: International Academy
of Cardiology, 12th World Heart Congress, New Trends in
Research, Diagnosis, and Treatment Vancouver, B.C., Canada,
July 16-19, 2005
Published in: The Journal of Heart Disease;
vol 4(1): 99, Abstract #394 |
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Impaired arterial
smooth muscle contractility plays a leading role in the development
of systemic hypertension. Structural changes, such as occlusive
atherosclerotic plaque formation, and functional changes,
such as hormonal disturbances, play roles in pathophysiological
mechanisms of altered arterial contractility. Accelerated
gel contraction was accompanied by elevated secretion of MMPs
into cell culture media. We found that purified polyphenols
and catechins counteracted SMC-dependent collagen gel contraction;
this gel relaxation effect was further enhanced by addition
of ascorbic acid and amino acids lysine, arginine, cysteine
and proline to green tea extract. A reduction in gel contraction
correlated with decreased MMP expression. These results are
significant as they indicated that nutrients can effectively
counteract angiotensin-mediated excessive stimulation of arterial
tissue contraction and have therapeutic potential in hypertension. |
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Extracellular
Matrix-Mediated Control of Smooth Muscle Cell Growth and
Migration by a Combination of Ascorbic Acid, Lysine, Proline
and Catechins
V. Ivanov, S. Ivanova, M.W. Roomi, A.
Niedzwiecki, M. Rath, Matthias Rath BV, Santa Clara, CA
Presented at: 75th European Atherosclerotic
Society Congress, Prague, Czech Republic, April 23-26, 2005.
Published in: Atherosclerosis, vol 6,
issue 1, 2005, p.18.
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Under conditions
of chronic essential nutrient deficiency, the extracellular
matrix (ECM) produced can support the development of atherosclerotic
lesions. We investigated the effect of nutrients essential
for collagen synthesis on ECM biological properties: SMC proliferation,
invasiveness and migration, and collagen type ratios. The
ECM deposited in the presence of a mixture of nutrients (ascorbate,
lysine, proline, and green tea extract) had improved strength
and integrity (increased collagen IV: I and chondroitin sulfate:
heparan) and significantly inhibited SMC migration up to 70%.
These results are important as they indicate that proper nutrient
supplementation can prevent the formation of atherosclerotic
lesions. |
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A
Nutrient Approach to Inhibition of Cardiovascular Disease
V. Ivanov, S. Ivanova, M.W. Roomi, A.
Niedzwiecki, M. Rath
Oral Presentation at: American Chemical
Society National Meeting, San Diego, CA, March 13-17, 2005
Published in: Book of Abstracts, 229th
ACS National Meeting, Abstract # 60 |
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Every year
over 12 million people worldwide die of the results of atherosclerosis,
heart infarctions, and strokes. Conventional treatment focuses
on symptoms, but does not address the cellular cause of cardiovascular
disease – chronic deficiency of essential nutrients.
The nutrient mixture of ascorbic acid, lysine, proline and
green tea extract inhibited the key steps in atherosclerotic
plaque progression:
• Inflammation - Inhibits secretion
of inflammatory mediators and Inhibits attraction of monocytes
• Oxidation - Protects LDL from free radicals
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SMC growth and invasion - Direct and ECM-mediated inhibitory
effects
• ECM production - Promotes anti-atherogenic
changes in ECM composition and quantity |
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Bioflavonoids
Effectively Inhibit Smooth Muscle Cell-Mediated Contraction
of Collagen Matrix Induced by Angiotensin II (2004)
Ivanov V, Ivanova S, Roomi MW, Niedzwiecki
A, Rath M.
Presented at: 5th Annual Conference
on Arteriosclerosis, Thrombosis, and Vascular Biology, San
Francisco, CA, May 6-8, 2004.
Published in: 5th Annual Conference
on Arteriosclerosis, Thrombosis, and Vascular Biology Abstracts,
p. 20, Abstract #67.
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Plant-derived
bioflavonoids have been recognized to support arterial wall
structural integrity and interfere with a variety of pro-atherosclerotic
stimuli. This study examined the effect of various bioflavonoids
on angiotensin II-stimulated contraction by human aortic smooth
muscle cells (SMC) embedded in a three-dimensional collagen
matrix. Bioflavonoid inhibition of SMC contraction was found
to be dependent upon structural characteristics with EGCG
and quercetin showing the greatest inhibition at 97% and 120%,
respectively. These results are significant since they imply
that plant derived bioflavonoids have a great potential in
controlling hypertension by counteracting pathophysiological
effects of angiotensin. |
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Nutrient
Synergy – A Mixture of Ascorbic Acid, Lysine, Proline,
Arginine, Cysteine and Green Tea Extract Suppresses Autocrine
Inflammatory Response in Cultured Human Aortic Smooth Muscle
Cells (2003)
Ivanov V, Ivanova S, Roomi MW, Netke
SP, Niedzwiecki A, Rath M.
Presented at: 3rd World Congress on
Heart Disease, Washington, DC, July 12-15, 2003.
Published in: The Journal of Heart Disease,
Vol. 3, No. 1., July 2003, p. 54, Abstract #214.
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Anti-inflammatory
effects of EF exceeded the sum of actions of its individual
components. Data confirms that Nutrient Synergy, a mixture
of ascorbic acid, tea phenolics, and selected amino acids,
has a strong inhibitory potential against vascular cell inflammatory
responses to pathogenic stimuli. |
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Anti-Atherogenic
Effects of a Mixture of Ascorbic Acid, Lysine, Proline,
Arginine, Cysteine and Green Tea Phenolics in Human Aortic
Smooth Muscle Cells (2003)
Ivanov V, Ivanova S, Roomi MW Netke
SP, Niedzwiecki A, Rath M.
Presented at: 12th International Congress
on Cardiovascular Pharmacotherapy, Barcelona, Spain, May
7-10, 2003.
Published in: The Abstract Book by Prous
Science, 2003.
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In this study,
the synergistic anti-atherogenic effects of nutrients such
as ascorbic acid, lysine, proline, arginine, cysteine, and
epigallocatechin gallate (from green tea extract) were investigated
in cultured human aortic smooth muscle cell (SMC) by measuring
SMC growth rate, invasiveness, matrix metalloproteinase-2
(MMP-2) expression, and secretion of monocyte chemoattractant
protein-1 (MCP-1) and interleukin-6 (IL-6). By inhibiting
the atherogenic response of vascular smooth muscle cells to
pathological stimuli, Nutrient Synergy blocks the development
of atherosclerotic lesions. |
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Enhancement
of Cardio-Protective Effects and Attenuation of Adverse
Effects of Female Sex Hormones on Cultured Human Vascular
Smooth Muscle Cells by a Combination of Ascorbic Acid, Lysine,
Proline, Arginine, Cysteine and Epigallocatechin Gallate
(2003)
Ivanov V, Ivanova S, Roomi MW, Netke
SP, Niedzwiecki A, Rath M.
Presented at: 6th European Congress
of Endocrinology, Lyon, France, April 26-30, 2003.
Published in: The Abstract Book by the
European Federation of Endocrine Societies, Abstract #P0750,
2003.
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Large numbers
of menopausal women are using hormone replacement therapy
(HRT) for counteracting such adverse effects as hot flashes
and loss of bone mass. However, HRT has been recently shown
to have major adverse effects on cardiovascular health. When
tested in an experimental system of cultured human vascular
smooth muscle cells, female sex hormones produced diverse
effects on atherogenic changes in smooth muscle cells properties
such as pathologically increased growth rate and invasiveness
and excessive production of extracellular matrix components
and inflammatory SW. Nutrient Synergy enhanced the cardio-protective
action of female sex hormones and counteracted their adverse
effects in these experimental conditions, suggesting that
it has great potential as an adjunctive therapeutic agent
in inhibiting adverse effects and enhancing the cardio-protective
effects of female sex hormones in HRT. |
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Cellular Medicine
Research Breakthroughs in the Control of Diseases: Report
on Scientific Research (2003)
Ivanov V, Ivanova S, Roomi MW, Netke
SP, Niedzwiecki A, Rath M.
Presented at: 21st Century Medical Advances
in the Treatment of Cancer and Aging, Miami, FL, March 22-24,
2003.
Published in: Conference Proceedings.
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Current concepts
of pathogenesis in cardiovascular disease focus on elevated
plasma risk factors damaging the vascular wall and, thereby,
initiating atherogenesis. These concepts, however, are not
adequate in explaining many aspects of the pathomechanisms
of this disease. A new scientific rationale1 proposes that
a chronic deficiency of vitamins and other essential nutrients
impairs vascular cell functions. This results in morphological
and physiological changes in the vascular wall, which trigger
repair mechanisms comprising deposition of systemic plasma
factors as well cellular responses and lead to atherosclerosis.
This scientific rationale has been confirmed in various experimental
settings, including the results of a study in 55 patients
with various degrees of coronary calcifications identified
by UCT. The study confirmed that a specific nutritional supplement
program optimizing vascular wall functions is able to halt
the progression of early stages of vascular calcifications
and even, in individual cases, reverse coronary heart disease2.
The results imply that CHD is a preventable and controllable
condition through natural means. |
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A
Randomized, Double-Blind Placebo-Controlled Clinical Study
Documents the Benefits of a Vitamin Program as an Adjunct
Therapy in Patients With Paroxysmal Atrial Arrhythmia (2003)
Niedzwiecki A, Rath M.
Presented at: 14th Madrid Arrhythmia
Meeting, Madrid, Spain, February 27-28, 2003.
Published in: Conference Proceedings.
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The
combination of vitamins, minerals and amino acids used in
this study was effective in reducing the apparent clinical
symptoms of arrhythmia over a 6-month period. This vitamin
program can be implemented in clinical practice as a safe
and effective therapy in patients suffering from arrhythmia. |
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