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Scientific Presentations in Cancer
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Antineoplastic
Activity of a Novel Nutrient Mixture on Malignant Retinoblastoma
Cell Line Y79
- NEW -
M. Waheed Roomi, Vadim Ivanov, Aleksandra
Niedzwiecki, Matthias Rath.
Dr Rath Research Institute, Santa Clara, California, USA
Presented at: International Congress of
Toxicology, Montreal, Canada, July 15-19, 2007
Published in: International Congress
of Toxicology, Carcinogenesis, July 2007, Abstract #PW2.042,page
84
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Currently there
is no effective treatment for retinoblastoma (Rb), a malignant
tumor of the retina which affects children under the age of
six. Rb metastasizes to extraocular organs including bone,
lung and brain. We investigated the in vitro antineoplastic
activity of a non-toxic nutrient mixture (NM) consisting of
lysine, proline, ascorbic acid and green tea extract that
has shown anticancer activity against a number of cancer cell
lines on human malignant retinoblastoma cell line Y79. Rb
cells exhibited 25% toxicity to 10-100 µg/ml NM and
profound toxicity at 500 and 1000 µg/ml NM. Zymography
demonstrated production of only MMP-2. NM at 10-100 µg/ml
had no effect on MMP-2, but completely blocked it at 500 µg/ml.
PMA treatment did not induce MMP-9. Interestingly, Rb cells
were not invasive through Matrigel. These results are significant
as they suggest NM may have therapeutic potential in the treatment
of retinoblastoma. |
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Pattern
of MMP-2 and MMP-9 Secretion in Human Cancer Cell Lines
- NEW -
M.W. Roomi, J. Monterrey, V. Ivanov,
A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, Oncology Division, 1260 Memorex
Drive, Santa Clara, CA
Presented at: 98th Annual Meeting of the
AACR, Los Angeles, CA, April 14-18, 2007
Published in: Proceedings of the 98th Annual
Meeting of the AACR, Abstract #559, p 114 |
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Matrix metalloproteinases
(MMPs), a class of zinc dependent neutral proteinases encoded
by a multigene family, have been implicated in cancer. MMP-2
and MMP-9 secretion is elevated in several types of human
cancers and their elevated expression has been associated
with poor prognosis. Expression of MMPs is highly regulated
by cytokines and signal transduction pathways, including those
activated by PMA. In this study, we examined the effect of
PMA on MMP-2 and MMP-9 secretion in several human cancer cell
lines. Based on the MMP-2 and MMP-9 secretion with and without
PMA treatment, the various human cancer cell lines fell into
three groups: 1) Those secreting only MMP-2 and not stimulated
by PMA; 2) Those secreting MMP-9 only, which was either stimulated
further by PMA or unaffected by PMA, and 3) Those secreting
both MMP-2 and MMP-9, with PMA stimulation of only MMP-9.
The finding that irrespective of MMP-2 secretion, only MMP-9
levels are increased, suggest that this enzyme might play
a more vital role in invasion and metastasis and may be a
valuable indicator of the prognosis of the pathological conditions. |
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Inhibition
of 7,12-Dimethylbenzanthracene-Induced Skin Tumor by a Nutrient
Mixture
- NEW -
M.W. Roomi, N.W. Roomi, V. Ivanov, A.
Niedzwiecki and M. Rath
Dr. Rath Research Institute, Oncology Division, 1260 Memorex
Drive, Santa Clara, CA
Presented at: Annual Meeting of the AACR,
Los Angeles, CA, April 14-18, 2007
Published in: Proceedings of the 98th Annual
Meeting of the AACR, Abstract #2634, p 231 |
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The annual
incidence of skin cancer, the most common of all human cancers,
is increasing each year. Nearly half of Americans who live
to the age of 65 will develop skin cancer. We examined the
effect of a unique nutrient mixture (NM) that contains lysine,
proline, ascorbic acid and green tea extract on the development
of skin cancer induced by topical application of 7,12-dimethylbezanthracene
(DMBA) in female SENCAR mice. Groups of mice were treated
with NM by diet and or topically or with saline. NM significantly
inhibited DMBA-induced skin tumors. Topical NM treatment prior
to DMBA application was the most effective treatment (reducing
the incidence of skin tumors by 86%), followed by 2-week pretreatment
with a diet enriched with NM (69% reduction in tumors) and
treatment with dietary NM during the study (59% reduction
in tumors). These results are significant as they indicate
that NM has strong potential as a therapeutic regimen for
prevention and treatment of skin cancer. |
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A
Novel Nutrient Mixture Inhibits Cell Proliferation, MMP
Secretion and Invasion in HepG2 Hepatoma Cell Line
- NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: 46th Annual Meeting of the
Society of Toxicology; Charlotte, NC; March 25-29, 2007
Published in: The Toxicologists (suppl
Toxicological Sciences), Abstract #610, pg 126, 2007 |
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To provide
a better understanding of the preventive effect of NM (a unique
nutrient mixture containing lysine, proline, ascorbic acid
and green tea extract) on liver cancer, we examined NM on
HepG2 cell proliferation, MMP secretion, invasion, morphology,
and apoptosis. NM showed dose-dependent inhibition of MMP-2,
with virtual total inhibition at 500 µg/ml and dose-dependent
inhibition of Matrigel invasion and migration of human hepatocellular
carcinoma HepG2 with 97% inhibition at 500 µg/ml and
total blockage at 1000 µg/ml. HepG2 cells showed no
morphological changes below 500 µg/ml and obvious apoptosis
at higher concentrations. Moderate apoptosis was observed
at 100 µg/ml NM and profound at 500 and 1000 µg/ml
NM. These results are significant since they suggest NM has
therapeutic potential in liver cancer by inducing apoptosis
and inhibiting invasive parameters. |
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A
Novel Nutrient Mixture Induces Apoptosis in Human Prostate
Cancer Cell Line Du-145
- NEW -
M.W. Roomi, N.W. Roomi, V. Ivanov, A.
Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: GTCbio Apoptosis in Drug
Discovery; San Diego, CA; March 22-23, 2007
Published in: Proccedings of the GTCbio
Conference on Apoptosis in Drug Discovery |
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A novel antineoplastic
agent, a nutrient mixture (NM) containing lysine, proline,
ascorbic acid and green tea extract demonstrates a broad spectrum
of antitumor activity against a number of human cancer cell
lines. We investigated whether the cytotoxic effect of NM
on DU145 was consistent with its apoptotic effect on these
cells. DU-145 cells treated with NM at 500 and 1000 µg/ml
demonstrated profound toxicity and obvious apoptotic cells
morphologically. Using caspases kit, slight apoptosis was
observed at 100 µg, moderate at 500 and significant
at 1000 µg/ml concentrations. The results are significant
as they suggest NM as a therapeutic agent for prostate cancer,
the major cancer affecting males and second most deadly cancer
in the United States. |
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Apoptosis
of Human Cervical and Ovarian Cancer Cell Lines Induced
by a Novel Nutrient Mixture
- NEW -
M.W. Roomi, N.W. Roomi, V. Ivanov, A.
Niedzwiecki and M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: GTCbio Apoptosis in Drug
Discovery; San Diego, CA; March 22-23, 2007
Published in: Proceedings of the GTCbio
Conference on Apoptosis in Drug Discovery |
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A unique antitumor
agent, a nutrient mixture (NM) containing lysine, proline,
ascorbic acid and green tea extract has demonstrated anticarcinogenic
activity against a number of human cancer cell lines, including
ovarian and cervical cancer. We investigated whether the antitumor
effect of NM on cervical and ovarian cancer cell lines was
due to apoptosis. NM was toxic to cervical (Hela) cells but
did not affect ovarian cancer (SK-OV-3) cells. However, morphology
and caspase apoptosis assays indicated that NM induced apoptosis
in both cells lines – moderate at 100 µg/ml and
profound at 1000 µg/ml. These results are significant
as they suggest that NM has therapeutic potential in treatment
of ovarian and cervical cancer. |
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Suppression
of Growth In Vivo and In Vitro of Murine B16FO Melanoma
Cells by a Novel Nutrient Mixture
- NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki
and M. Rath
Presented at: 46th
Annual Meeting of The American Society for Cell Biology,
San Diego December
9-13, 2006
Published in: 46th Annual Meeting of The
American Society for Cell Biology, San Diego, December 9-13,
2006 Proceedings, Abstract # 1280. |
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Melanoma is
a very serious and highly metastatic form of skin cancer,
which causes the most skin cancer-related deaths. In its advanced
stages melanoma is resistant to existing therapies. We investigated
the effect of a unique nutrient mixture (NM) containing lysine,
proline, ascorbic acid and green tea extract on murine B16FO
melanoma cells in vitro and also in vivo by injecting these
melanoma cells under the skin of nude mice. After 4 weeks
of supplementation with NM growth of melanoma tumors in mice
was inhibited by 50%. Melanoma cells exposed to 500 and 1000
mg/ml NM concentration in vitro, exhibited 50% toxicity over
the control. At these concentrations a moderate and extensive
apoptosis (natural cell death) was observed respectively.
These results are significant as they suggest NM as a therapeutic
agent for melanoma. |
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Marked
Inhibition of Tumor Growth, MMP Secretion and Invasion by
a Nutrient Mixture on Head and Neck Squamous Carcinoma Cell
Line FaDu: In Vitro and In Vivo studies
- NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, Oncology, 1260 Memorex Drive,
Santa Clara, CA 95050
Presented at: 18th EORTC-NCI-AACR Symposium
on Molecular Targets and Cancer Therapeutics, November 7-10,
2006, Prague, Czechoslovakia
Published in: The European Journal of Cancer
2006, vol 4(12), abstract #520 |
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Head and neck
squamous cell carcinomas (HNSCC), the sixth most common malignancies
in the United States, are known for their aggressive growth
and propensity to invade and metastasize. We investigated
the effect of a novel nutrient mixture (NM) containing ascorbic
acid, lysine, proline, and green tea extract, shown to have
potent antitumor effects on many cancer cell lines, on the
growth of human HNSCC xenografts in athymic nude mice, as
well as investigating this cell line in vitro, evaluating
viability, MMP secretion, invasion and morphology. NM inhibited
the growth of tumors by 50% and exhibited dose-response toxicity
to cells in vitro, with 50% toxicity at 1000 µg/ml. In addition,
NM inhibited the invasive parameters of MMP secretion and
Matrigel invasion by cells in a dose-dependent manner with
total block of MMPs at 1000 µg/ml and invasion at 500 µg/ml.
These results are significant as they suggest that NM, a relatively
safe therapeutic agent, has great potential in treatment of
HNSCC in suppressing tumor growth and metastasis. |
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Inhibition
of Human Neuroblastoma Cell Line SK-N-MC In Vivo
and In Vitro By a Novel Nutrient Mixture
- NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Presented at: 21st Annual Meeting of the
ISBTC, October 26-29, 2006, Los Angeles, CA
Published in: Proceedings of the 21st Annual
Meeting of the ISBTC, abstract #116 |
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This study
shows that specific nutrients combined in a synergy have a
potential to curb neuroblastoma tumor growth in vivo by about
30%. In addition, by inhibiting secretion of MMPs, the enzymes
essential for degradation of collagen and connective tissue
surrounding all cancer cells, they can curb cancer spread
in the body. Prevention of ECM degradation by inhibition of
MMP activity has been shown to be a promising therapeutic
target in cancer. Our unique nutrient mixture (NM) containing
lysine, proline, ascorbic acid and green tea extract inhibited
the secretion of both MMP-2 and MMP-9 with their total blockage
at NM concentration of 100 mg/ml. These results are significant
as they demonstrate therapeutic potential in treatment of
neuroblastoma by safe and effective micronutrient synergy. |
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In Vitro Inhibition of MMPS, Invasion and Growth
of Human Fanca and Fancc Lymphoblasts by a Unique Nutrient
Mixture
- NEW -
M.W.Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, Oncology, 1260 Memorex Drive,
Santa Clara, CA 95050
Presented at: 18th Annual Fanconi Anemia
Research Fund Scientific Symposium, October 19-22, 2006,
Bethesda, Maryland
Published in: 18th Annual Fanconi Anemia
Research Fund Scientific Symposium proceedings, pg 64 |
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Fanconi anemia
(FA) is a rare genetic disorder with high propensity to development
of cancer. Biological agents that prevent extracellular matrix
degradation by MMPs have been shown to be promising therapeutic
approaches to cancer A micronutrient mixture (NM) containing
ascorbic acid, lysine, proline and green tea extract has shown
significant anticancer activity against a number of cancer
cell lines. We investigated the effect of NM on human FANCA
and FANCC lymphoblasts for viability, MMP secretion and invasion.
NM inhibited MMP secretion and Matrigel invasion in FANCA,
and invasion and proliferation in FANCC lymphoblasts, suggesting
NM has a potential therapeutic use in the treatment strategy
in FA neoplasia. |
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Inhibition
of Hemangioendothelioma In Vivo and Invasion and
Growth In Vitro by a Unique Nutrient Mixture
- NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki
and M. Rath
Dr. Rath Research Institute, Oncology Division, Santa Clara,
CA 95050
Presented at: 47th Annual Meeting of the
American College of Nutrition, October 5-8, 2006, Reno,
NV
Published in: Journal of the American College
of Nutrition vol 25(5), October 2006, abstract #23 |
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Current treatment
for infantile hemangioendothelioma includes toxic agents such
as systemic corticosteroids, vincristine, and interferon-alpha.
We investigated the effect of a unique non-toxic micronutrient
mixture (NM) containing lysine, proline, ascorbic acid and
green tea extract, which has shown significant anti-angiogenic
and anti-tumor effect against a number of cancer cell lines,
on nude mice injected subcutaneously with EOMA cells. In addition,
we tested NM in vitro on EOMA cells evaluating its effect
on cell proliferation, invasion, and cell morphology. NM inhibited
the growth of tumors by 50% and exhibited dose response toxicity
and inhibition of EOMA Matrigel invasion. EOMA cell morphology
was not affected even at highest concentration tested. These
results are significant as they indicate NM is a potential
non-toxic alternative agent for treatment of infantile hemangioendothelioma. |
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Inhibitory
Effect of a Novel Nutrient Mixture on MMP Secretion and
Invasion on Human Thyroid Cancer Cell Line SW 579: In
Vitro Study
M.W. Roomi, N.W. Roomi, V. Ivanov,
A. Niedzwiecki and M. Rath
Dr. Rath Research Institute, Oncology Division, Santa Clara,
CA 95050
Presented at: AICR International Research
Conference on Food, Nutrition and Cancer, July 13-14, 2006,
Washington DC
Published in: AICR International Research
Conference on Food, Nutrition and Cancer Poster Abstract
Book, Abstract #42 |
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Mortality from
thyroid cancer, the most common type of endocrine malignancy,
results mainly from the tumor invading local tissues and metastasizing
to vital organs such as the liver, lung, and brain. Metastasis
depends upon degradation of the ECM and is mediated by MMPs.
A novel nutrient mixture (NM) containing ascorbic acid, lysine,
proline and green tea extract has exhibited MMP inhibition
and significant anticancer activity against a number of cancer
cell lines. Our in vitro study on the effect of NM on thyroid
cancer cells SW 579 demonstrated inhibition of invasive parameters
such as MMP secretion and Matrigel invasion, without toxicity
to cells, indicating NM has therapeutic potential in treatment
of thyroid cancer. |
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A
Nutrient Mixture Consisting of Lysine, Proline, Ascorbic
Acid and Green Tea Extract Inhibit Lung Metastasis by B16FO
Melanoma Cells in Mice
M.W. Roomi, N.W. Roomi, V. Ivanov,
A. Niedzwiecki, M. Rath
Dr. Rath Research Institute, Oncology Division, Santa Clara,
CA 95050
Presented at: 97th Annual Meeting of the
AACR, Washington DC, April 1-5, 2006
Published in: Proceedings of the 97th Annual
Meeting of the AACR, Abstract #1903 |
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A
Nutrient Mixture Consisting of Lysine, Proline, Ascorbic
Acid and Green Tea Extract Inhibit Lung Metastasis by B16FO
Melanoma Cells in Mice
M.W. Roomi, N.W. Roomi, V. Ivanov,
A. Niedzwiecki, M. Rath
Dr. Rath Research Institute, Oncology Division, Santa Clara,
CA 95050
Presented at: 97th Annual Meeting of the
AACR, Washington DC, April 1-5, 2006
Published in: Proceedings of the 97th Annual
Meeting of the AACR, Abstract #1903 |
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Tumor metastasis
is a major reason for treatment failure in cancer patients.
Metastatic malignant melanoma is an extremely aggressive cancer
with no current viable therapy. The primary objective of our
study was to investigate whether a unique non-toxic nutrient
mixture (NM) that has shown potent anti-tumor effects in a
number of cancer cell lines, could inhibit experimentally
induced lung metastasis of melanoma cells in C57BL/6 mice.
Intravenous injection of melanoma cells into C57BL/6 mice
has been shown to result in pulmonary metastasis, providing
an excellent model to assess test agents on metastasis. This
study demonstrated the effectiveness of NM in halting metastasis
of B16FO cells in mice, especially when delivered by iv or
ip. Furthermore, pre-incubation of tumor cells with NM for
18 hrs prior to injection into the mice completely prevented
the development of lung tumors in these mice. These results
are significant since they clearly demonstrate the anti-metastatic
potential of NM, a non-toxic nutrient mixture. |
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Antileukemic
Effect of a Novel Nutrient Mixture on Human JURKAT T Cells
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: FASEB, San Francisco, CA,
April 1-5, 2006
Published in: The FASEB Journal, Abstract
#107 |
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Cellular production
of matrix metalloproteinases contributes to tumor progression,
invasion and metastasis. We investigated whether cellular
invasion and production of MMPs by a leukemia cell line, Jurkat
T, can be affected by a unique nutrient mixture (NM) consisting
primarily of lysine, proline, ascorbic acid and green tea
extract. We have demonstrated previously (Harakeh et al. 2006)
that this nutrient mixture was effective in inducing apoptosis
in this and other leukemia cell lines. In addition, it has
been shown to exhibit anti-tumor activity both in vitro and
in vivo against a number of cancer cell lines. NM exhibited
antiproliferative effect on human acute Jurkat T cells at
500?g/ml, and dose-dependent inhibition of MMP-9 secretion
and Matrigel invasion, with total blockage of MMP-9 secretion
at 500 ?g/ml NM and of invasion at 1000 ?g/ml NM. These results
are significant as they confirm previous data and indicate
that NM is potentially a promising therapeutic agent for acute
leukemia. |
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Inhibition
of MMP Activity and Cell Invasion by a Novel Nutrient Mixture
on Glioma Cell Line A-172: in vitro Study
M.W. Roomi, V. Ivanov, A. Niedzwiecki
and M. Rath
Dr. Rath Research Institute, Oncology Division, 1260 Memorex
Drive, Santa Clara, CA 95050
Presented at: 45th
Annual Meeting of The American Society for Cell Biology,
San Francisco, December 10-14, 2005
Published in: 45th
Annual Meeting of The American Society for Cell Biology,
San Francisco, December 10-14, 2005 Proceedings, Abstract
#L461
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Five-year survival
rates for brain tumors are among the lowest of all cancers
(pancreatic and lung being the shortest). The median survival
of patients with malignant glioma is 14-36 weeks, depending
upon treatment regimens, mainly due to invasion into the surrounding
brain. A unique non-toxic nutrient mixture (NM) consisting
of lysine, proline, ascorbic acid and green tea extract, shown
to exhibit anti-tumor effects in a number of cancer cell lines
both in vitro and in vivo, significantly inhibited glioma
cell MMP secretion and invasion in vitro, suggesting NM as
a potentially effective treatment strategy to block cell invasion
of gliomas. |
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in
vivo and in vitro Antitumor Effect of Ascrobic
Acid, Lysine, Proline, and Green Tea Extract on Human Melanoma
a2058 Cells
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, Cancer Division, Santa Clara,
CA 95050, USA
Presented at: AACR-NCI-EORTC
International Conference on Molecular Targets and Cancer
Therapeutics: Discovery, Biology, and Clinical Applications,
November 14-18, 2005
Published in: Program and
Proceedings of AACR-NCI-EORTC International Conference on
Molecular Targets and Cancer Therapeutics: Discovery, Biology,
and Clinical Applications, Abstract #B133
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Due to metastasis,
melanoma causes the most skin cancer-related deaths. MMPs,
VEGF, ki 67 (proliferative protein), and constituents of ECM
play critical roles in neoplastic invasion and metastasis.
We found that nutrient supplemented (NM 0.5%) nude mice with
melanoma xenografts developed significantly smaller tumors
than did the control group of nude mice accompanied by reduced
tumor tissue MMP-9 and VEGF levels and reduced mitotic index.
These findings were supported by in vitro studies on melanoma
A2058 cells that demonstrated NM inhibition of MMP-9 and MMP-2
secretion and dose-dependent inhibition in Matrigel invasion
by these cells. Morphology was not affected even at the highest
concentration of NM. These results are important as they indicate
tumor growth and metastatic parameters are reduced with use
of the non-toxic nutrient mixture, offering a potentially
safe and effective therapeutic agent in treatment of melanoma. |
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Anti-Cancer
Activity of a Nutrient Mixture in Malignant Leukemia Cell
Line P-388 In Vitro
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, Cancer Division,
Santa Clara, CA 95050
Presented at: International Society for
Biological Therapy of Cancer, Nov 10-13, 2005, Alexandria,
VA.
Published in: International Society
for Biological Therapy of Cancer Proceedings, Abstract
#96. |
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Leukemia, a
disorder of bone marrow characterized by unrestrained white
blood cell proliferation, results in formation of metastatic
colonies and penetration through matrix barriers and blood
vessel walls. Degradation and invasion through matrix barriers
and vessel walls is mediated by secretion of MMPs. A nutrient
mixture (NM) containing lysine, proline, ascorbic acid and
green tea extract exhibited significant inhibition of Matrigel
invasion (with total block at 1000 _g/ml) and significant
antiproliferative effect at 500 _g/ml (70%) and 1000 _g/ml
(90%). These results are important as they indicate NM is
a promising therapeutic agent for leukemia. |
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Regulation
of MMP-2 and MMP-9 Expression in Childhood Osteosarcoma
by Inducers and Inhibitors #95
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, Cancer Division, Santa Clara,
CA 95050, USA
Presented at: International
Society for Biological Therapy of Cancer, Nov 10-13, 2005,
Alexandria, VA
Published in: International
Society for Biological Therapy of Cancer Proceedings,
Abstract #95 |
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Osteosarcoma,
the most frequent malignant bone tumor of childhood, is highly
invasive, leading to pulmonary metastases and eventual death,
despite conventional chemotherapy and surgical treatments.
Degradation of the extracellular matrix, which leads to invasion
and metastasis occurs secondary to secretion of matrix metalloproteinases
(MMPs), in particular MMP-2 and MMP-9. In this study the inducers
and inhibitors of MMP-2 and –9 by osteosarcoma U-20S
cells were studied in vitro. MMP-2 secretion by osteosarcoma
cell line U-2OS was not influenced by PMA, cytokines and LPS.
However, the induction of MMP-9 was associated with the inducer
used, strongest with PMA, followed by cytokines, and least
with LPS. MMP-2 and MMP-9 were down regulated with inhibitors.
These results are important since they indicate that inhibitors
such as EGCG, doxycycline and others have a significant inhibitory
effect on osteosarcoma MMP-2 and MMP-9 secretion in vitro,
and thus have potential in treatment of osteosarcoma. |
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Inhibitory
Effect of a Novel Mixture Containing Ascorbic Acid, Lysine,
Proline and Green Tea Extract on Critical Parameters in
Cancer Progression
M.W. Roomi, V. Ivanov, T. Kalinovsky,
A. Niedzwiecki, M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: 10th World Congress on
Advances in Oncology and 8th International Symposium on
Molecular Medicine, Crete, Greece, October 13-15, 2005.
Published in: International Journal
of Molecular Medicine, abstract #121, page S10. |
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egradation
of extracellular matrix (ECM) is a hallmark of tumor invasion,
metastasis and angiogenesis. Based on a multitargeted approach
to cancer by using natural substances to control ECM stability
and enhancing its strength we developed a novel formulation
(NM) of lysine, proline, ascorbic acid and green tea extract
that has shown significant anti-cancer activity against a
number of cancer cell lines. Using various in vitro
and in vivo experimental models, we found that NM
significantly inhibited angiogenesis and metastasis of various
cancer cell lines. These results suggest that the nutrient
mixture (NM) has strong therapeutic potential treating various
cancers by blocking angiogenesis and tumor invasion and metastasis. |
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A
Novel Nutrient Mixture as an Antileukemic Agent with Matrix
Metalloproteinase and Invasion Inhibitory Activities
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: 2nd International Tumor
Metabolism Summit, Genova, Italy, October 7-8, 2005
Published in: 2nd International Tumor
Metabolism Summit Proceedings |
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Acute myeloid
leukemia (AML), the most common leukemia in adults, is characterized
by uncontrolled proliferation of myeloid blasts or lymphoid
cell lines. Although chemotherapy and supportive management
have resulted in high remission rates, fewer than 50% of young
patients and 10%-15% of patients over 60 years are cured of
their disease. We found that the nutrient mixture (NM) of
ascorbic acid, lysine, proline, and green tea extract demonstrated
a potent synergistic anticancer effect by inhibiting human
promyelocytic leukemia cell HL-60 proliferation, MMP activity,
and invasion through Matrigel in vitro. Our results
are significant as they indicate that this nutrient mixture
is a promising non-toxic therapeutic agent for acute myeloid
leukemia. |
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Down
Regulation of Matrix Metalloproteinases and Inhibition of
Cell Invasion by a Novel Nutrient Mixture in Human FA Fibroblast
Cell Lines: in vitro Study
M.Waheed. Roomi, Vadim. Ivanov, Aleksandra.
Niedzwiecki and Matthias Rath
Matthias Rath Research Institute, Cancer Division, Santa
Clara, CA
Presented at: 17th Annual Fanconi Anemia
Scientific Symposium, Geneva, Switzerland, September 29-October
2, 2005
Published in: 17th Annual Fanconi Anemia
Scientific Symposium Proceedings, Poster #110 |
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Fanconi anemia
(FA is characterized by a high probability of developing malignancies
at an early age. Extracellular matrix (ECM) matrix metalloproteinases
(MMPs) produced by tumor and stromal cells, in particular
MMP-2 and MMP-9, play a key role in tumor invasion and metastasis.
Our in vitro studies demonstrated that the nutrient
mixture (NM) of ascorbic acid, lysine, proline, and green
tea extract inhibited FA cell MMP expression and invasion
- important parameters for cancer prevention. These results
are significant as they indicate that the nutrient mixture
has strong potential as a non-toxic treatment strategy in
FA neoplasia. |
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Novel
Anticarcinogenic Effect of a Nutrient Mixture: Inhibition
of MMPs, Invasion and Growth of Human Rhabdomyosarcoma in
vitro
Roomi MW, Ivanov V, Niedzwiecki A and
Rath M
Matthias Rath Research Institute, Cancer Division, Santa
Clara, CA 95050
Presented at: 46th Annual Meeting of
the American College of Nutrition, Charleston, South Carolina,
Sept 22-25, 2005
Published in: Journal of the American
College of Nutrition, vol 24(5), Oct 2005, abstract #75 |
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Rhabdomyosarcoma
is the most common soft tissue sarcoma of mesenchymal origin
in infants and children. We investigated the effect of a nutrient
mixture (NM) of lysine, proline, ascorbic acid and green tea
extract, shown previously to have potent antitumor effects
on various cancer cell lines, on MMP expression, invasion
and growth of pediatric rhabdomyosarcoma. Matrix metalloproteinases
(MMPs) play a crucial role in tumor cell invasion, metastasis
and angiogenesis, due to their ability to digest ECM and basement
membrane. NM inhibited MMP-2 and MMP-9 secretion in a dose-dependent
fashion, with virtual total inhibition at 500 ?g/ml NM and
blocked Matrigel invasion at 10, 50, 100 and 500 ?g/ml by
70%, 80%, 90% and 100% respectively. H&E staining did
not indicate any change even at the highest concentration.
Our results are significant as they indicate that the relatively
non-toxic NM has strong potential in rhabdomyosarcoma treatment. |
|
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Studies
on the Bioenhancing Effects of Red Onions and Other Nutrients
on the Absorption of Epigallocatechin Gallate from Green
Tea Extract in Human Volunteers
Anup Kale1, Sonia Gawande1, Swati Kotwal1,
Shrirang Netke2, Waheed Roomi2, Vadim Ivanov2, Aleksandra
Niedzwiecki2 and Matthias Rath2
1 Post Graduate Department of Biochemistry, University of
Nagpur, Nagpur, M.S. 440033, India
2 Dr Matthias Rath Institute of Cellular Research,1260 Memorex
Drive, Santa Clara, CA 95050, US
Presented at: 2nd International Conference
on Tumor Progression & Therapeutic Resistance, Boston,
September 18-20, 2005
Published in: 2nd International Conference
on Tumor Progression & Therapeutic Resistance Proceedings,
page 89. |
| |
Consumption
of high amounts of tea has been associated with lower incidence
of stomach cancer. Furthermore, In vitro and in vivo studies
have demonstrated the anti-cancer activity of green tea, black
tea and their active constituents. However, plasma levels
of EGCG after human consumption of green tea products indicate
poor absorption of EGCG. We sought to determine if EGCG absorption
could be enhanced with red onion (quercetin) supplementation.
The results demonstrated that increasing the amount of quercetin
given along with EGCG could increase absorption of EGCG from
the intestine. Thus, the anticancer activity of the nutrient
mixture (EF) can be enhanced with quercetin intake by increasing
plasma levels of EGCG. |
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Inhibition
of Malignant Mesothelioma Cell Matrix Metalloproteinase
Production and Invasion by a Novel Nutrient Supplement
M.W. Roomi, V. Ivanov, A. Niedzwiecki
and M.Rath
Matthias Rath Research Institute, Cancer Division, Santa
Clara, CA
Presented at: Directions and Needs in
Asbestos Research, University of Montana, July 28-29, 2005
Published in: Conference proceedings |
| |
Malignant mesothelioma
(MM) is an asbestos-associated cancer with no known cure.
It is a highly aggressive tumor causing profound morbidity
and nearly universal mortality. Extracellular matrix (ECM)
matrix metalloproteinases (MMPs) produced by tumor and stromal
cells play a key role in tumor invasion and metastasis. The
nutrient mixture (NS) of ascorbic acid, lysine, proline, arginine
and green tea extract significantly inhibited MM cell MMP
expression and invasion- both important parameters for cancer
prevention, suggesting this nutrient mixture has strong therapeutic
potential for treatment of malignant mesothelioma. |
|
|
Inhibition
of Cell Invasion and MMP Production by a Nutrient Mixture
in Malignant Liposarcoma Cell Line SW-872
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: International Research
Conference on Food, Nutrition, and Cancer, Washington, DC,
July 14-15, 2005
Published in: Poster Abstract Book,
Abstract # 36
|
| |
Liposarcoma,
a fat cell malignancy, commonly metastasizes (usually to lungs
and liver). Overall 5-year survival rate of patients with
deep high-grade liposarcoma is less than 50%. Extracellular
matrix (ECM) matrix metalloproteinases (MMPs) produced by
tumor and stromal cells play a key role in tumor invasion
and metastasis. The nutrient mixture (NM) of lysine, proline,
ascorbic acid and green tea extract significantly inhibited
liposarcoma cell growth, MMP expression and invasion - important
parameters for cancer prevention. These results are significant
as they indicate that the nutrient mixture has therapeutic
potential as a non-toxic treatment strategy for liposarcoma. |
|
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Anti-Angiogenic
Activity of a Novel Nutrient Mixture of Lysine, Proline,
Ascorbic Acid and Green Tea Extract.
M.W. Roomi, N.W. Roomi, V. Ivanov, A.
Niedzwiecki and M. Rath
Matthias Rath Research, Cancer Division, 1260 Memorex Drive,
Santa Clara, CA 95050
Presented at: 1st International Symposium
on Cancer Metastasis and Lymphovascular System, San Francisco,
CA, April 28-30, 2005
Published in: 1st International
Symposium on Cancer Metastasis and Lymphovascular System:
Basis for Rational Therapy, Abstract #18
|
| |
Endothelial
cell proliferation, migration and subsequent invasion of the
underlying stoma is critical to angiogenesis. We studied the
effects of a nutrient mixture (NM) of lysine, proline, arginine,
ascorbic acid, and green tea extract on angiogenic parameters
of human umbilical endothelial cells (HUVECs). NM showed dose-dependent
inhibition of MMP-2, Matrigel invasion, cell migration, and
vascular tube with complete blockage of all parameters at
500 µg/ml. NM did not significantly decrease cell proliferation
or morphology. The results are important as they indicate
that NM is an effective anti-angiogenic agent with no apparent
cell toxicity. |
|
|
Anti-Angiogenic
and Antimetastatic Activities of a Novel Nutrient Formulation
of Lysine, Proline, Ascorbic Acid, and Green Tea Extract
M.W. Roomi, V. Ivanov, A. Niedzwiecki
and M. Rath
Matthias Rath Research, Cancer Research Division,
1260 Memorex Drive, Santa Clara, CA 95050
Presented at: 96th Annual Meeting of the
AACR, Anaheim, CA, April 16-20, 2005.
Published in: Proceedings of the 96th
Annual Meeting of the AACR, Abstract #2991. |
| |
Angiogenesis,
a process of abnormal new blood vessel development from pre-existing
capillaries, is a main prerequisite for tumor growth. It is
a complex multi-step process that plays an important role
in tumor growth, invasion and metastasis. We designed a novel
formulation (NM) of ascorbic acid, lysine, proline and green
tea extract that has shown significant anti-cancer activity
against a number of cancer cell lines. The aim of the present
study was to determine whether NM exhibits anti-angiogenic
and antimetastatic effects using in vitro and in vivo experimental
models. Since angiogenesis depends on the interaction between
tumor cells and endothelial cells, we sought to determine
the effect of NM on both cell types. |
|
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Chemoprevention
with Chlorophyllin by Inhibiting MMP Expression and Invasiveness:
In Vitro Studies
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Presented at: XXV International Congress
of Physiological Sciences, March 31-April 5, 2005, San Diego
Published in: The FASEB Journal, Abstracts,
Abstract #860.11
|
| |
Fibrosarcoma
is an aggressive and highly metastatic cancer with poor prognosis
associated with both the aggressive metastatic spread characteristic
of this cancer and the lack of efficacy in current treatment
modalities to prevent, counteract or slow tumor progression.
Chlorophyllin (CHL), a water-soluble derivative of chlorophyll,
used as a food colorant and over-the counter medicine, exhibited
potent antimetastatic activity on human fibrosarcoma HT180cells
in vitro; it inhibited Matrigel invasion and MMP expression
- both important parameters for cancer prevention. |
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A
Novel Approach to Inhibition of Cancer Development by Nutrient
Supplement
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Oral Presentation at: American Chemical
Society National Meeting, San Diego, CA, March 13-17, 2005
Published in: Book of Abstracts, 229th
ACS National Meeting, Abstract #86 |
| |
Cancer, one
of the most dreaded diseases, is the second leading cause
of death in the United States. The standard treatment protocols
of chemotherapy and radiation are toxic to normal cells and
potentially enhance metastasis. In contrast, the nutrient
mixture of ascorbic acid, lysine, proline and green tea extract
addresses different aspects of cancer development:
•
Inhibits tumor growth/ proliferation
• Impairs invasion
• Inhibits metastasis
• Inhibits angiogenesis |
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Suppression
of MMP Expression and Invasion of Human Cervical Cancer
Cell Lines Hela and DoTc2 4510 by Nutrients
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Presented at: 44th Annual Meeting of
the Society of Toxicology, New Orleans, March 6-10, 2005.
Published in: Proceedings of the 44th
Annual Meeting of the Society of Toxicology, Abstract #149
|
| |
Cervical cancer
is the seventh most common cancer worldwide and the second
most common cancer in women. Untreated dysplasia and carcinoma
in situ leads to 30% to 40% invasion within ten years. We
studied the synergistic effect of a unique nutrient formulation
containing lysine, proline, arginine, ascorbic acid, and epigallocatechin
gallate on metastatic parameters on human cervical cancer
cells such as effect on MMP-2 and –9 expression and
cancer cell invasive potential. Our results demonstrated complete
inhibition of Hela MMP-2 expression at 1000 µg/ml and
MMP-9 at 500 µg/ml, and of Matrigel invasion at 500
µg/ml. NM demonstrated complete inhibition of cervical
DoTc2 4510 cell expression of MMP-9 at 500 µg/ml and
of invasion at 1000 µg/ml. These findings are significant
as they suggest that the nutrient mixture would be an effective
and safe therapeutic regimen for treatment of cervical cancer.
|
|
|
Suppression
of Human Rheumatoid Synovial Fibroblast MMP-2 Expression
and Marix Invasion by Nutrient Synergy
M.W.
Roomi, V. Ivanov, A. Niedzwiecki and M. Rath
Matthias Rath Research, Cancer Research Division,
Santa Clara, CA 95050
Presented at: 9th World Congress of the
Osteoarthritis Research Society International, Chicago,
Illinois, December 2-5, 2004.
Published in: Osteoarthritis and Cartilage,
Abstract #193. |
| |
In patients
with rheumatoid arthritis and other inflammatory diseases,
MMPs are expressed in synovial membranes and have been implicated
in synovial tissue invasion into adjacent cartilage and bone.
Standard anti- arthritic drugs, used for prolonged periods,
have been associated with serious side effects. We studied
the in vitro effect of a mixture of nutrients containing ascorbic
acid, lysine, arginine and epigallocatechin gallate on human
synovial fibroblast growth, MMP expression, and invasive potential.
Our results demonstrated complete inhibition of MMP-2 expression
and invasive potential of synovial fibroblasts at 500 _g/ml,
suggesting this nutrient mixture has excellent potential as
a safe anti-inflammatory agent. |
|
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Nutrient
Synergy - an Important Inhibitor of MMP-2 Expression and
Invasion by Human Ovarian Cancer Cell Line SK-OV-3
M.W.
Roomi, V. Ivanov, A. Niedzwiecki and M. Rath
Matthias Rath Research., Cancer Research Division,
Santa Clara, CA 95050
Presented at: International Society for
Biological Therapy of Cancer, San Francisco, November 4-7,
2004.
Published in: Journal of Immunotherapy
(Official journal of International Society for Biological
Therapy of Cancer) volume 27, number 6, page S8, November/December
2004. |
| |
Due to high
metastasis rates, ovarian cancer is the deadliest of the gynecologic
cancers and the fifth leading cause of cancer death among
U.S. women. We studied the synergistic effect of a unique
nutrient formulation containing lysine, proline, arginine,
ascorbic acid, and epigallocatechin gallate on metastatic
parameters on human ovarian cancer cells SK-OV-3, such as
effect on MMP-2 and –9 expression and cancer cell invasive
potential. Our results demonstrated complete inhibition of
MMP-2 expression at 50 mg/ml and of Matrigel invasion at 1000
mg/ml. These findings are significant as they suggest that
nutrient synergy would be an effective and safe therapeutic
regimen for treatment of ovarian cancer. |
|
|
Naturally Produced
Extracellular Matrix Inhibits Growth Rate and Invasiveness
of Human Osteosarcoma Cancer Cells
V. Ivanov,
V. Ivanova, M.W. Roomi, A. Niedzwiecki and M. Rath
Matthias Rath BV, Santa Clara, CA 95050
Presented at: American College of Nutrition
Symposium on Advances on Clinical Nutrition, Long Beach,
CA, September 30-October 3, 2004
Published in: ACN Symposium on Advances
on Clincal Nutrition Proceedings, Abstract #49,
pg 465
|
| |
Enhanced supplementation
with certain naturally occurring nutrients and vitamins has
been associated with a reduction in occurrence and progression
of human cancer. The exact mechanisms of this action are still
under investigation. Extracellular matrix (ECM) plays a key
role in the development of cancer. Therefore, we studied whether
nutrients could exert anti-cancer effects through alteration
of ECM biological properties. The results demonstrated that
the ECM produced by normal fibroblasts treated with a mixture
of ascorbic acid, lysine, proline, arginine, cysteine, and
green tea polyphenols significantly reduced the growth rate
and invasive activity of osteosarcoma cells in contrast to
the non-supplemented control. We conclude that anti-cancer
effects of nutrients involve beneficial changes in ECM biological
properties. |
|
|
Nutrition Synergy
Inhibits MMP Expression and Invasion Potential of Human
Lung Carcinoma Cell Line A-549
M.W.
Roomi,V. Ivanov, A. Niedzwiecki and M. Rath
Matthias Rath Research, Cancer Division, Santa Clara, CA
95050
Presented at: American College of Nutrition
Symposium on Advances on Clinical Nutrition, Long Beach,
CA, September 30-October 3, 2004
Published in: ACN Symposium on Advances
in Clinical Nutrition Program, Abstract #50, pg 465. |
| |
Lung cancer,
the most common form of cancer diagnosed in the United States,
has one of the lowest survival rates of any cancer due to
a high metastatic rate and poor results with standard treatment
regimens. There is compelling evidence that invasion and metastasis
depend on degradation of the extracellular matrix (ECM), particularly
by MMP –2 and –9 expression. In this study of
the effect of a unique combination of lysine, proline, arginine,
ascorbic acid, and epigallocatechin gallate on human lung
cancer cell A-549 MMP expression and matrix invasion in vitro,
we found complete inhibition of MMP-2 and –9 expression
and Matrigel invasion at 500 _g/ml NM. These results suggest
that the nutrient combination tested has great potential as
a safe but effective therapeutic regimen for this highly aggressive
cancer. |
|
|
Antitumor Effect
of Nutrient Synergy on Human Bladder Cancer Cell Lines T-24
M.W.
Roomi, V. Ivanov, A. Niedzwiecki and M. Rath
A. Niedzwiecki
Matthias Rath Research., Cancer Research Division,
Santa Clara, CA 95050
Presented at: Mayo Clinic Conference on
Dietary Factors and Cancer Prevention, Rochester, MN, September
23-25, 2004.
Published in: Dietary Factors and Cancer
Prevention: Current Premises and Future Promises, Abstract
#25, pg 51 |
| |
Bladder cancer,
a cancer that develops mainly in older adults, is the fourth
most frequently diagnosed cancer in men. Once the cells have
metastasized, prognosis is poor. We investigated the antitumor
effect of a unique nutrient formulation containing lysine,
proline, arginine, ascorbic acid, and epigallocatechin gallate
on human bladder cancer cells T-24 in cell culture, by measuring
critical parameters in cancer development and spread, such
as MMP expression and cancer cell invasive potential. Our
results showed significant inhibition of invasion and of MMP-2
and –9 expression, which suggest that this nutrient
combination is potentially an excellent candidate for prevention
and treatment of bladder cancer. |
|
|
Anticancer
Effect of Lysine, Proline, Arginine, Ascorbic Acid and Green
Tea Extract on Human Renal Adenocarcinoma Line 786-0 (2004)
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Presented at: Third
International Kidney Cancer Symposium, Chicago, Illinois,
November 12-14 2004.
Published in: The Third International
Kidney Cancer Symposium proceedings, Abstract #39, pg 39.
|
| |
Renal adenocarcinoma,
the 17th most frequent cancer worldwide, is associated with
good prognosis if treated when still localized to the kidney;
however, once metastasized, prognosis is poor. We investigated
the effect of a unique nutrient formulation (NM) of lysine,
proline, arginine, ascorbic acid, and epigallocatechin gallate
on its effect in vitro on modulation of metastatic parameters
in human renal adenocarcinoma cell line 786-0. NM significantly
inhibited MMP expression and Matrigel invasion in a dose-dependent
fashion, with total inhibition of MMP-2 at 500 µg/ml,
MMP-9 at 100 µg/ml and invasion at 1000 µg/ml.
These results indicate NM has great potential in therapeutic
use for treatment of renal carcinoma. |
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In
vitro Expression of MMP-2 and MMP-9 by Human Renal
Cell Carcinoma with Modulation by Phorbol Ester, TNF-alpha,
Il-1 beta and Lipopolysaccharides (2004)
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Presented at: Third International Kidney
Cancer Conference, Chicago, Illinois, November 12-14, 2004.
Published in: Third International
Kidney Cancer Symposium proceedings, Abstract #40, pg 40.
|
| |
Renal cell
carcinoma invasion is associated with its ability to degrade
the extracellular matrix by local production of gelatinase
enzymes (MMP-2 and MMP-9). Although many studies on renal
carcinoma have demonstrated the importance of MMPs, very little
information is currently known regarding the effect of inhibitors
and inducers of MMPs. We studied the effect of inducers (PMA,
TNF-alpha, IL-1 beta, and LPS) and inhibitors (EGCG, doxycycline,
actinomycin D, cyclohexamide, retinoic acid, dexamethasone
and H-7) on RCC 786-0 MMP expression. Our results indicate
that MMP-2 expression was not influenced by inducers such
as PMA, cytokines (IL-1b, TNFa), and LPS; the induction of
MMP-9 was associated with the specific inducer used: highest
with PMA, intermediate with cytokines, and none with LPS.
The inhibitors tested affected both MMP-2 and MMP-9 expression.
Further studies are in progress to confirm the role of MMP-9
on Matrigel invasion using PMA, cytokines and LPS. |
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Anti-Angiogenic
Effect of Nutrient Synergy on Human Synovial Sarcoma Cell
Line SW 982 (2004)
Roomi MW, Ivanov V, Niedzwiecki A, Rath
M.
Presented at: International Research
Conference on Food, Nutrition and Cancer in Washington DC,
July 15-16, 2004.
Published in: Conference Proceedings,
pg. 31, Abstract #29.
|
| |
Standard treatment
of synovial sarcoma, a soft tissue with a high (50%) metastatic
rate has met with poor results. In this study, we investigated
the inhibitory effect of a unique nutrient mixture (NS) containing
lysine, proline, arginine, ascorbic acid, and epigallocatechin
gallate on metastatic potential of human synovial sarcoma
cells SW 982, by measuring MMP expression and Matrigel invasion
in cell culture. We found NS to inhibit the expression of
both MMP-2 and -9 in a dose-dependent fashion with virtual
total inhibition of MMP-2 at 500 µg/ml and MMP-9 at
50 µg/ml concentration of NS. The invasion of human
synovial sarcoma cells through Matrigel was significantly
reduced at 500 µg/ml (79%) and totally inhibited at
1000 µg/ml concentration of the synergistically acting
nutrient mixture (p<0.0001). These results are important
as they demonstrate that NS is an excellent candidate for
treatment of synovial sarcoma by inhibiting critical steps
in metastasis. |
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Anti-Proliferative
Effects of Antioxidants Using HTLV-1 Positive and Negative
Malignant T-Cells (2004)
Harakeh
SM, Diab-Assaf M, Niedzwiecki A, Khalife J, Abu-El-Ardat K,
Roomi MW, Rath M. American University of Beirut, Beirut, Lebanon.
Presented at: 104th General Meeting of
the American Society for Microbiology, New Orleans, Louisiana,
May 23-27, 2004.
Published in: American Society for Microbiology
Final Program, p. 143, Abstract #T-021. |
| |
Adult T-cell
leukemia (ATL) is an aggressive malignancy caused by HTLV-1,
for which currently there is no proven therapy. Nutrients,
such as ascorbic acid, lysine, and EGCG used individually
and in combination with other nutrients (e.g., proline and
N-Acetyl-L-Cysteine), were effective in inhibiting the growth
of virus-infected and virus-negative leukemia cell types.
These nutrients were used in concentrations that did not affect
the viability of normal cells, but were effective in killing
leukemia cells. Our study results confirm our previous findings
that a specific combination of nutrients can be a powerful
tool in suppressing cancer cell growth and viability, including
blood cancers such as ATL. |
|
|
Inhibition
of N-Methyl-N-Nitrosourea-Induced Mammary Tumors by Nutrient
Synergy – A Novel Anti-Cancer Agent (2004)
Roomi
MW, Roomi NW, Ivanov V, Niedzwiecki A, Rath M.
Presented at: 95th Annual Meeting of American
Association for Cancer Research, Orlando, FL, March 27-31,
2004.
Published in: 95th Annual Meeting of American
Association for Cancer Research Proceedings, 45:881, March
2004. |
| |
Incidence and
mortality from breast cancer in women, the third most prevalent
cancer worldwide, is steadily increasing, especially in developed
societies. This study examined the synergistic effect of a
specific formulation of nutrients containing lysine, proline,
ascorbic acid and green tea extract on the growth of mammary
tumors induced in 50-day-old female Sprague-Dawley rats by
the carcinogen N-methyl-N-nitrosourea (MNU). Nutrient Synergy
significantly inhibited the incidence, as well as the growth,
of MNU-induced mammary tumors, indicating that it has strong
potential as a useful therapeutic regimen for inhibiting breast
cancer development. |
|
|
Inhibition
of Tumor Growth of Human Breast, Prostate, Colon and Melanoma
Cancer Xenografts by Nutrient Synergy in Nude Mice (2003)
Roomi MW, Roomi NW, Ivanov V, Netke SP,
Niedzwiecki A, Rath M.
Presented at: 43rd Annual Meeting of the
American Society for Cell Biology
Published in: The American Society for
Cell Biology 43rd Annual Meeting Abstract, p. 921, Abstract
#B132, December 17, 2003. |
| |
This study
demonstrated the synergistic anticancer effects of lysine,
proline, arginine, ascorbic acid and EGCG (from green tea
extract) on human breast, colon, prostate, melanoma, fibrosarcoma,
and synovial sarcoma cancer cell growth in nude mice without
any adverse effects. Nude mice are used since they are athymic,
and thus are unable to mount most types of immune responses,
including the ability to kill malignant cells by a cell-mediated
immune response. The results of this study imply that Nutrient
Synergy has great potential as a safe and effective therapeutic
regimen for cancer treatment. |
|
|
Anti-Tumor
Effect of Nutrient Synergy: A Novel MMP Inhibitor in Pancreatic
Cancer Cell Line MIA PaCa-2 (2003)
Roomi
MW, Ivanov V, Niedzwiecki A, Rath M.
Presented at: AACR-NCI-EORTC International
Conference on Molecular Targets and Cancer, Boston, November
17-21, 2003.
Published in: Program Proceedings of the
AACR-NCI-EORTC International Conference on Molecular Targets
and Cancer, p. 165, Abstract #B196. |
| |
Cancer of the
pancreas is a highly lethal disease with the poorest likelihood
of survival among all major malignancies; metastasis is associated
with more than 80% of the cases. This study demonstrated significant
inhibition of the metastatic parameters of MMP expression,
invasion, and angiogenesis, suggesting NS is an excellent
candidate for therapeutic use in the treatment of pancreatic
cancer. |
|
|
Nutrient
Synergy Counteracts Carcinogenic Activity of Estrogen in Cultured
Human Cancer Cells (2003)
Ivanov V, Roomi MW, Niedzwiecki A, Rath M.
Presented
at: American College of Nutrition, Nashville, Tennessee,
October 9-12, 2003.
Published
in: Journal of the American College of Nutrition, 22
(5):466, Abstract #44. |
| |
Recent clinical
studies have shown that hormone replacement therapy (HRT)
in menopausal women increases the risk of tumor development
in estrogen-sensitive tissues. In this study, estradiol stimulated
breast cancer (MCF-7) cell growth, MMP expression, matrix
invasion, and VEGF secretion (measure of angiogenesis) were
observed in culture. These pro-carcinogenic effects of estradiol
were significantly inhibited by Nutrient Synergy, suggesting
this is an excellent candidate for preventative and therapeutic
use in the treatment of estrogen-related breast cancer. |
|
|
Serum
Markers of the Liver, Heart, and Kidney and Lipid Profile
and Histopathology in ODS Rats Treated With Nutrient Synergy
(2003)
Roomi MW,
Ivanov V, Netke SP, Niedzwiecki A, Rath M.
Presented at: American College of Nutrition,
Nashville, Tennessee, October 9-12, 2003.
Published in: Journal of the American
College of Nutrition, 22 (5):477, Abstract #86. |
| |
These results
demonstrate that Nutrient Synergy has neither adverse effects
on vital organs nor on the associated serum enzymes, and i | |
